Transcriptional Landscape of Vero E6 Cells during Early Swine Acute Diarrhea Syndrome Coronavirus Infection. 2021

Siying Zeng, and Ouyang Peng, and Ruipu Sun, and Qiuping Xu, and Fangyu Hu, and Yan Zhao, and Chunyi Xue, and Yongchang Cao, and Hao Zhang
State Key Laboratory of Biocontrol, Life Sciences School, Sun Yat-sen University, Guangzhou 510006, China.

Swine acute diarrhea syndrome coronavirus (SADS-CoV) is a newly emerged and highly pathogenic virus that is associated with fatal diarrhea disease in piglets, causing significant economic losses to the pig industry. At present, the research on the pathogenicity and molecular mechanisms of host-virus interactions of SADS-CoV are limited and remain poorly understood. Here, we investigated the global gene expression profiles of SADS-CoV-infected Vero E6 cells at 12, 18, and 24 h post-infection (hpi) using the RNA-sequencing. As a result, a total of 3324 differentially expressed genes (DEG) were identified, most of which showed a down-regulated expression pattern. Functional enrichment analyses indicated that the DEGs are mainly involved in signal transduction, cellular transcription, immune and inflammatory response, and autophagy. Collectively, our results provide insights into the changes in the cellular transcriptome during early infection of SADS-CoV and may provide information for further study of molecular mechanisms.

UI MeSH Term Description Entries
D002522 Chlorocebus aethiops A species of CERCOPITHECUS containing three subspecies: C. tantalus, C. pygerythrus, and C. sabeus. They are found in the forests and savannah of Africa. The African green monkey is the natural host of SIMIAN IMMUNODEFICIENCY VIRUS and is used in AIDS research. African Green Monkey,Cercopithecus aethiops,Cercopithecus griseoviridis,Cercopithecus griseus,Cercopithecus pygerythrus,Cercopithecus sabeus,Cercopithecus tantalus,Chlorocebus cynosuros,Chlorocebus cynosurus,Chlorocebus pygerythrus,Green Monkey,Grivet Monkey,Lasiopyga weidholzi,Malbrouck,Malbrouck Monkey,Monkey, African Green,Monkey, Green,Monkey, Grivet,Monkey, Vervet,Savanah Monkey,Vervet Monkey,Savannah Monkey,African Green Monkey,Chlorocebus cynosuro,Green Monkey, African,Green Monkeys,Grivet Monkeys,Malbrouck Monkeys,Malbroucks,Monkey, Malbrouck,Monkey, Savanah,Monkey, Savannah,Savannah Monkeys,Vervet Monkeys
D005786 Gene Expression Regulation Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation. Gene Action Regulation,Regulation of Gene Expression,Expression Regulation, Gene,Regulation, Gene Action,Regulation, Gene Expression
D000073638 Alphacoronavirus A genus of the family CORONAVIRIDAE which causes respiratory or gastrointestinal disease in a variety of mammals. Human alphacoronaviruses include Human coronavirus 229E and NL63. All members share the identical core transcription regulatory sequences of 5’-CUAAAC-3’ and most have 1 to 2 ORFs downstream to the N protein gene. Bat coronavirus CDPHE15,Bat coronavirus HKU10,Miniopterus bat coronavirus 1,Miniopterus bat coronavirus HKU8,Mink coronavirus 1,Rhinolophus bat coronavirus HKU2,Scotophilus bat coronavirus 512,Alphacoronaviruses,Bat coronavirus CDPHE15s,CDPHE15, Bat coronavirus,HKU10, Bat coronavirus
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D014709 Vero Cells A CELL LINE derived from the kidney of the African green (vervet) monkey, (CHLOROCEBUS AETHIOPS) used primarily in virus replication studies and plaque assays. Cell, Vero,Cells, Vero,Vero Cell
D015203 Reproducibility of Results The statistical reproducibility of measurements (often in a clinical context), including the testing of instrumentation or techniques to obtain reproducible results. The concept includes reproducibility of physiological measurements, which may be used to develop rules to assess probability or prognosis, or response to a stimulus; reproducibility of occurrence of a condition; and reproducibility of experimental results. Reliability and Validity,Reliability of Result,Reproducibility Of Result,Reproducibility of Finding,Validity of Result,Validity of Results,Face Validity,Reliability (Epidemiology),Reliability of Results,Reproducibility of Findings,Test-Retest Reliability,Validity (Epidemiology),Finding Reproducibilities,Finding Reproducibility,Of Result, Reproducibility,Of Results, Reproducibility,Reliabilities, Test-Retest,Reliability, Test-Retest,Result Reliabilities,Result Reliability,Result Validities,Result Validity,Result, Reproducibility Of,Results, Reproducibility Of,Test Retest Reliability,Validity and Reliability,Validity, Face
D054884 Host-Pathogen Interactions The interactions between a host and a pathogen, usually resulting in disease. Host Pathogen Interaction,Host-Pathogen Relations,Pathogen-Host Interaction,Pathogen-Host Interactions,Host Pathogen Interactions,Host Pathogen Relations,Host-Pathogen Interaction,Host-Pathogen Relation,Interaction, Host Pathogen,Interaction, Host-Pathogen,Interaction, Pathogen-Host,Interactions, Host Pathogen,Interactions, Host-Pathogen,Interactions, Pathogen-Host,Pathogen Host Interaction,Pathogen Host Interactions,Pathogen Interaction, Host,Pathogen Interactions, Host,Relation, Host-Pathogen,Relations, Host-Pathogen
D059467 Transcriptome The pattern of GENE EXPRESSION at the level of genetic transcription in a specific organism or under specific circumstances in specific cells. Transcriptomes,Gene Expression Profiles,Gene Expression Signatures,Transcriptome Profiles,Expression Profile, Gene,Expression Profiles, Gene,Expression Signature, Gene,Expression Signatures, Gene,Gene Expression Profile,Gene Expression Signature,Profile, Gene Expression,Profile, Transcriptome,Profiles, Gene Expression,Profiles, Transcriptome,Signature, Gene Expression,Signatures, Gene Expression,Transcriptome Profile
D018352 Coronavirus Infections Virus diseases caused by the CORONAVIRUS genus. Some specifics include transmissible enteritis of turkeys (ENTERITIS, TRANSMISSIBLE, OF TURKEYS); FELINE INFECTIOUS PERITONITIS; and transmissible gastroenteritis of swine (GASTROENTERITIS, TRANSMISSIBLE, OF SWINE). Infections, Coronavirus,MERS (Middle East Respiratory Syndrome),Middle East Respiratory Syndrome,Coronavirus Infection,Infection, Coronavirus
D020869 Gene Expression Profiling The determination of the pattern of genes expressed at the level of GENETIC TRANSCRIPTION, under specific circumstances or in a specific cell. Gene Expression Analysis,Gene Expression Pattern Analysis,Transcript Expression Analysis,Transcriptome Profiling,Transcriptomics,mRNA Differential Display,Gene Expression Monitoring,Transcriptome Analysis,Analyses, Gene Expression,Analyses, Transcript Expression,Analyses, Transcriptome,Analysis, Gene Expression,Analysis, Transcript Expression,Analysis, Transcriptome,Differential Display, mRNA,Differential Displays, mRNA,Expression Analyses, Gene,Expression Analysis, Gene,Gene Expression Analyses,Gene Expression Monitorings,Gene Expression Profilings,Monitoring, Gene Expression,Monitorings, Gene Expression,Profiling, Gene Expression,Profiling, Transcriptome,Profilings, Gene Expression,Profilings, Transcriptome,Transcript Expression Analyses,Transcriptome Analyses,Transcriptome Profilings,mRNA Differential Displays

Related Publications

Siying Zeng, and Ouyang Peng, and Ruipu Sun, and Qiuping Xu, and Fangyu Hu, and Yan Zhao, and Chunyi Xue, and Yongchang Cao, and Hao Zhang
September 2022, Journal of virology,
Siying Zeng, and Ouyang Peng, and Ruipu Sun, and Qiuping Xu, and Fangyu Hu, and Yan Zhao, and Chunyi Xue, and Yongchang Cao, and Hao Zhang
January 2020, Frontiers in veterinary science,
Siying Zeng, and Ouyang Peng, and Ruipu Sun, and Qiuping Xu, and Fangyu Hu, and Yan Zhao, and Chunyi Xue, and Yongchang Cao, and Hao Zhang
October 2020, Proceedings of the National Academy of Sciences of the United States of America,
Siying Zeng, and Ouyang Peng, and Ruipu Sun, and Qiuping Xu, and Fangyu Hu, and Yan Zhao, and Chunyi Xue, and Yongchang Cao, and Hao Zhang
January 2024, Animals : an open access journal from MDPI,
Siying Zeng, and Ouyang Peng, and Ruipu Sun, and Qiuping Xu, and Fangyu Hu, and Yan Zhao, and Chunyi Xue, and Yongchang Cao, and Hao Zhang
July 2004, Journal of medical virology,
Siying Zeng, and Ouyang Peng, and Ruipu Sun, and Qiuping Xu, and Fangyu Hu, and Yan Zhao, and Chunyi Xue, and Yongchang Cao, and Hao Zhang
July 2005, The Journal of general virology,
Siying Zeng, and Ouyang Peng, and Ruipu Sun, and Qiuping Xu, and Fangyu Hu, and Yan Zhao, and Chunyi Xue, and Yongchang Cao, and Hao Zhang
December 2003, The Journal of general virology,
Siying Zeng, and Ouyang Peng, and Ruipu Sun, and Qiuping Xu, and Fangyu Hu, and Yan Zhao, and Chunyi Xue, and Yongchang Cao, and Hao Zhang
April 2005, Biochemical and biophysical research communications,
Siying Zeng, and Ouyang Peng, and Ruipu Sun, and Qiuping Xu, and Fangyu Hu, and Yan Zhao, and Chunyi Xue, and Yongchang Cao, and Hao Zhang
November 2025, Journal of virology,
Siying Zeng, and Ouyang Peng, and Ruipu Sun, and Qiuping Xu, and Fangyu Hu, and Yan Zhao, and Chunyi Xue, and Yongchang Cao, and Hao Zhang
November 2003, Journal of medical virology,
Copied contents to your clipboard!