Biomaterial Database

Carbocyclic glycinamide ribonucleotide is a substrate for glycinamide ribonucleotide transformylase. 1988

C A Caperelli, and M F Price

Institute for Cancer Research, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111.

The carbocyclic analog of glycinamide ribonucleotide has been synthesized from the racemic parent trihydroxy cyclopentyl amine (B.L. Kam and N.J. Oppenheimer (1981) J. Org. Chem. 46, 3268-3272). This analog was accepted as a substrate (Km = 18 microM, Vmax = 0.23 mM/min) by mammalian glycinamide ribonucleotide transformylase (EC 2.1.2.2) with an efficiency comparable to that of the natural substrate glycinamide ribonucleotide (Km = 10 microM, Vmax = 0.27 mM/min). For each molecule of 10-formyl-5,8-dideazafolate cosubstrate consumed, 0.92 molecule of N-formyl carbocyclic glycinamide ribonucleotide was produced in the enzymatic reaction, indicating a 1:1 stoichiometry. These studies afford the first alternate nucleotide substrate for glycinamide ribonucleotide transformylase and suggest that the ribose ring oxygen of glycinamide ribonucleotide is not critical for enzyme recognition and binding.

UI	MeSH Term	Description	Entries
D007700	Kinetics	The rate dynamics in chemical or physical systems.
D000217	Acyltransferases	Enzymes from the transferase class that catalyze the transfer of acyl groups from donor to acceptor, forming either esters or amides. (From Enzyme Nomenclature 1992) EC 2.3.	Acyltransferase
D012265	Ribonucleotides	Nucleotides in which the purine or pyrimidine base is combined with ribose. (Dorland, 28th ed)	Ribonucleoside Phosphates,Ribonucleotide,Phosphates, Ribonucleoside
D013379	Substrate Specificity	A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts.	Specificities, Substrate,Specificity, Substrate,Substrate Specificities
D051026	Phosphoribosylglycinamide Formyltransferase	An enzyme that catalyzes the transfer of a formyl group from N10-formyltetrahydrofolate to N1-(5-phospho-D-ribosyl)glycinamide to yield N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide and tetrahydrofolate. It plays a role in the de novo purine biosynthetic pathway.	2-Amino-N-Ribosylacetamide 5'-Phosphate Transformylase,5'-Phosphoribosylglycinamide Transformylase,GAR Formyltransferase,GAR Transformylase,GARTFase,Glycinamide Ribonucleotide Transformylase,Glycinamideribotide Transformylase,2 Amino N Ribosylacetamide 5' Phosphate Transformylase,5' Phosphoribosylglycinamide Transformylase,5'-Phosphate Transformylase, 2-Amino-N-Ribosylacetamide,Formyltransferase, GAR,Formyltransferase, Phosphoribosylglycinamide,Ribonucleotide Transformylase, Glycinamide,Transformylase, 2-Amino-N-Ribosylacetamide 5'-Phosphate,Transformylase, 5'-Phosphoribosylglycinamide,Transformylase, GAR,Transformylase, Glycinamide Ribonucleotide,Transformylase, Glycinamideribotide
D019877	Hydroxymethyl and Formyl Transferases	Enzymes that catalyze the transfer of hydroxymethyl or formyl groups. EC 2.1.2.	Formyl Transferase,Formyltransferase,Hydroxymethyl Transferase,Hydroxymethyl and Formyl Transferase,Hydroxymethyltransferase,Transhydroxymethylase,Formyl Transferases,Formyltransferases,Hydroxymethyl Transferases,Hydroxymethyltransferases,Transformylase,Transformylases,Transhydroxymethylases,Transferase, Formyl,Transferase, Hydroxymethyl,Transferases, Formyl,Transferases, Hydroxymethyl