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The ability of the strain Bacillus thuringiensis var. subtoxicus to produce extracellular ribonuclease (ribonuclease Bt) was studied. It was found that the culture medium possesses a RNA-depolymerizing activity whose maximum is observed 4-5 hours after the beginning of the linear growth phase. A three-step chromatography of the culture extract on phosphocellulose resulted in a homogeneous enzyme with a molecular mass of 12000 Da. The enzyme showed the maximum activity towards RNA at pH 8.5, catalyzed the hydrolysis of polyribonucleotides and guanosine-2',3'-cyclophosphate. Hence, the enzyme can be related to base-nonspecific cyclizing ribonucleases showing the guanylic specificity towards nucleoside-2',3'-cyclophosphates.
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D L Liakhov, and
V O Rechinskiĭ, and
M Ia Karpeĭskiĭ
January 1992,
Molekuliarnaia biologiia,
N K Chepurnova, and
D L Liakhov, and
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M Ia Karpeĭskiĭ
September 1993,
Bioorganicheskaia khimiia,
N K Chepurnova, and
D L Liakhov, and
V O Rechinskiĭ, and
M Ia Karpeĭskiĭ
January 1992,
Nauchnye doklady vysshei shkoly. Biologicheskie nauki,
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Prikladnaia biokhimiia i mikrobiologiia,
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September 2000,
Bioorganicheskaia khimiia,
N K Chepurnova, and
D L Liakhov, and
V O Rechinskiĭ, and
M Ia Karpeĭskiĭ
January 1969,
Biokhimiia (Moscow, Russia),
N K Chepurnova, and
D L Liakhov, and
V O Rechinskiĭ, and
M Ia Karpeĭskiĭ
January 1998,
Mikrobiologiia,
N K Chepurnova, and
D L Liakhov, and
V O Rechinskiĭ, and
M Ia Karpeĭskiĭ
May 1981,
Biokhimiia (Moscow, Russia),
N K Chepurnova, and
D L Liakhov, and
V O Rechinskiĭ, and
M Ia Karpeĭskiĭ
January 1994,
Mikrobiologiia,
N K Chepurnova, and
D L Liakhov, and
V O Rechinskiĭ, and
M Ia Karpeĭskiĭ
November 1993,
Bioorganicheskaia khimiia,
