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The collisionally activated decomposition of [M + H]+ ions, generated by fast atom bombardment (FAB) of glutathione conjugates, has been studied by tandem mass spectrometry (MS/MS) using hybrid sector/quadrupole instruments. Abundant fragments of diagnostic utility were present in the daughter ion spectra. Common fragmentation modes were observed but their relative importance was strongly dependent on the nature of the conjugated species. As an example of a general approach to the characterization of glutathione conjugates in biological samples, the acetaminophen-glutathione conjugate was identified in rat bile, following coadministration of (2H0)- and (2H3)acetaminophen, using the experimental sequence: (i) conventional FAB mass spectrometric analysis, (ii) MS/MS using constant neutral loss (129 u) scanning to identify parent ions corresponding to glutathione conjugates, (iii) MS/MS to yield daughter ion spectra of parents so identified and corresponding to (2H0)- and (2H3)-labeled conjugates.
P E Haroldsen, and
M H Reilly, and
H Hughes, and
S J Gaskell, and
C J Porter
September 1987,
Biomedical & environmental mass spectrometry,
P E Haroldsen, and
M H Reilly, and
H Hughes, and
S J Gaskell, and
C J Porter
January 1986,
Drug metabolism and disposition: the biological fate of chemicals,
P E Haroldsen, and
M H Reilly, and
H Hughes, and
S J Gaskell, and
C J Porter
April 1990,
Biomedical & environmental mass spectrometry,
P E Haroldsen, and
M H Reilly, and
H Hughes, and
S J Gaskell, and
C J Porter
October 1988,
Biomedical & environmental mass spectrometry,
P E Haroldsen, and
M H Reilly, and
H Hughes, and
S J Gaskell, and
C J Porter
November 2001,
Journal of mass spectrometry : JMS,
P E Haroldsen, and
M H Reilly, and
H Hughes, and
S J Gaskell, and
C J Porter
January 1989,
Journal - Association of Official Analytical Chemists,
P E Haroldsen, and
M H Reilly, and
H Hughes, and
S J Gaskell, and
C J Porter
March 1989,
Biomedical & environmental mass spectrometry,
P E Haroldsen, and
M H Reilly, and
H Hughes, and
S J Gaskell, and
C J Porter
May 1999,
Biochimica et biophysica acta,
P E Haroldsen, and
M H Reilly, and
H Hughes, and
S J Gaskell, and
C J Porter
October 1988,
Biomedical & environmental mass spectrometry,
P E Haroldsen, and
M H Reilly, and
H Hughes, and
S J Gaskell, and
C J Porter
October 1990,
Steroids,
