Deepening the ideological and political construction of curriculum and carrying out the fundamental task of cultivating people with morality are the important requirements of education reform and talent cultivation in the new era. Microbiology Experiment is an important basic course and core practice course of Bioengineering, Pharmaceutical Engineering, Food Science and Engineering, et al. In order to give full play to the education function of Microbiology Experiment, this article deeply developed the ideological elements contained in the curriculum referring to the guidelines for the construction of ideological and political courses in institutions of higher education. And the article explored the ideological and political reform of Microbiology Experiment from three aspects: teaching content reform, teaching method innovation and improvement of teachers' ideological and political construction ability. Strive to integrate the value shaping, knowledge transference and ability training, cultivate high-quality professionals with firm ideals and beliefs.