Biomaterial Database

31P NMR relaxation time studies of 2,3-diphosphoglycerate in solution and intact erythrocytes. 1988

M Christensen, and J P Jacobsen

Department of Chemistry, Odense University, Denmark.

The 31P T1 spin-lattice relaxation times and nuclear Overhauser effects of 3-P and 2-P in 2,3-diphosphoglycerate (2,3-DPG) have been measured in pure water solutions and in suspensions of intact erythrocytes. It was found that extremely careful purification of the water solutions from paramagnetic impurities was necessary in order to obtain reproducible results. The dominant relaxation mechanism in purified samples was shown to be the dipole-dipole interaction. Contributions from the chemical-shift anisotropy mechanism were demonstrated to be important at higher magnetic field strengths. Based upon the measurements in water solutions and intact erythrocyte suspensions it was concluded that there could be observed no significant influence of the oxygenation state of hemoglobin on the 31P T1 values of 2,3-DPG.

UI	MeSH Term	Description	Entries
D009682	Magnetic Resonance Spectroscopy	Spectroscopic method of measuring the magnetic moment of elementary particles such as atomic nuclei, protons or electrons. It is employed in clinical applications such as NMR Tomography (MAGNETIC RESONANCE IMAGING).	In Vivo NMR Spectroscopy,MR Spectroscopy,Magnetic Resonance,NMR Spectroscopy,NMR Spectroscopy, In Vivo,Nuclear Magnetic Resonance,Spectroscopy, Magnetic Resonance,Spectroscopy, NMR,Spectroscopy, Nuclear Magnetic Resonance,Magnetic Resonance Spectroscopies,Magnetic Resonance, Nuclear,NMR Spectroscopies,Resonance Spectroscopy, Magnetic,Resonance, Magnetic,Resonance, Nuclear Magnetic,Spectroscopies, NMR,Spectroscopy, MR
D004163	Diphosphoglyceric Acids	Glyceric acids where two of the hydroxyl groups have been replaced by phosphates.	Bisphosphoglycerates,Acids, Diphosphoglyceric
D004912	Erythrocytes	Red blood cells. Mature erythrocytes are non-nucleated, biconcave disks containing HEMOGLOBIN whose function is to transport OXYGEN.	Blood Cells, Red,Blood Corpuscles, Red,Red Blood Cells,Red Blood Corpuscles,Blood Cell, Red,Blood Corpuscle, Red,Erythrocyte,Red Blood Cell,Red Blood Corpuscle
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D012996	Solutions	The homogeneous mixtures formed by the mixing of a solid, liquid, or gaseous substance (solute) with a liquid (the solvent), from which the dissolved substances can be recovered by physical processes. (From Grant & Hackh's Chemical Dictionary, 5th ed)	Solution
D014867	Water	A clear, odorless, tasteless liquid that is essential for most animal and plant life and is an excellent solvent for many substances. The chemical formula is hydrogen oxide (H2O). (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)	Hydrogen Oxide
D019794	2,3-Diphosphoglycerate	A highly anionic organic phosphate which is present in human red blood cells at about the same molar ratio as hemoglobin. It binds to deoxyhemoglobin but not the oxygenated form, therefore diminishing the oxygen affinity of hemoglobin. This is essential in enabling hemoglobin to unload oxygen in tissue capillaries. It is also an intermediate in the conversion of 3-phosphoglycerate to 2-phosphoglycerate by phosphoglycerate mutase (EC 5.4.2.1). (From Stryer Biochemistry, 4th ed, p160; Enzyme Nomenclature, 1992, p508)	2,3-Bisphosphoglycerate,2,3-DPG,2,3-Diphosphoglyceric Acid,2,3-Diphosphoglycerate, (D)-Isomer,Glycerate 2,3-Bisphosphate,2,3 Bisphosphoglycerate,2,3 Diphosphoglycerate,2,3 Diphosphoglyceric Acid,2,3-Bisphosphate, Glycerate