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BACKGROUND Canine embryo cryopreservation and subsequent transfer are relevant in the use of reproductive technologies. OBJECTIVE The purpose of this study is the identification and quantification of the gene expression BAX and Bcl2, AQP3, Na+/K+ ATPase alpha-1 and beta-1 and LIFr in canine embryos obtained in vivo and after freezing. METHODS For the collection of embryos, the bitches were identified at pro-estrous until the detection of 80-90% superficial cells. After that, they were artificially inseminated with fresh semen. The embryos were collected after ovariohysterectomy. RNA was extracted and amplified, and embryos were randomly distributed into fresh (Fr) and frozen/thawed (Ft) groups. RESULTS Eighteen blastocysts were collected from three bitches. Genes BAX, AQP3 and LIFr did not differ among the studied groups. CONCLUSIONS We suggest, through these results, that the genes BAX, Bcl2, AQP3, Na + / K + ATPase alpha-1 and beta-1 and LIFr were expressed in canine blastocysts collected in vivo and after slow freezing cryopreservation.
C Renato de Freitas Guaitolini, and
R R D Maziero, and
A Cesar de Souza Castilho, and
A A P Derussi, and
R Volpato, and
C L Ackermann, and
L M Matsubara, and
M D Lopes
November 2012,
Reproduction (Cambridge, England),
C Renato de Freitas Guaitolini, and
R R D Maziero, and
A Cesar de Souza Castilho, and
A A P Derussi, and
R Volpato, and
C L Ackermann, and
L M Matsubara, and
M D Lopes
January 1994,
Acta veterinaria Scandinavica,
C Renato de Freitas Guaitolini, and
R R D Maziero, and
A Cesar de Souza Castilho, and
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C L Ackermann, and
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M D Lopes
October 1991,
Theriogenology,
C Renato de Freitas Guaitolini, and
R R D Maziero, and
A Cesar de Souza Castilho, and
A A P Derussi, and
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C L Ackermann, and
L M Matsubara, and
M D Lopes
March 1992,
Theriogenology,
C Renato de Freitas Guaitolini, and
R R D Maziero, and
A Cesar de Souza Castilho, and
A A P Derussi, and
R Volpato, and
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L M Matsubara, and
M D Lopes
March 2015,
Theriogenology,
C Renato de Freitas Guaitolini, and
R R D Maziero, and
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R Volpato, and
C L Ackermann, and
L M Matsubara, and
M D Lopes
June 1985,
Experientia,
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L M Matsubara, and
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Biology of reproduction,
C Renato de Freitas Guaitolini, and
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A A P Derussi, and
R Volpato, and
C L Ackermann, and
L M Matsubara, and
M D Lopes
March 2008,
Animal reproduction science,
C Renato de Freitas Guaitolini, and
R R D Maziero, and
A Cesar de Souza Castilho, and
A A P Derussi, and
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C L Ackermann, and
L M Matsubara, and
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C Renato de Freitas Guaitolini, and
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M D Lopes
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