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Histone-gene expression was studied during the cell cycle of continuously dividing HeLa S3 cells and after stimulation of confluent monolayers of WI-38 human diploid fibroblasts to proliferate. The presence of histone-mRNA sequences was assayed by hybridization to a 3H-labelled single-stranded DNA complementary to histone-mRNA molecules. In HeLa S3 cells histone mRNA sequences were found in the nucleus and associated with polyribosomes during S-phase, but not during G1-phase. Transcripts of S-phase chromatin contained histone-mRNA sequences, but those of G1-phase chromatin did not. Similarly, in WI-38 cells association of histone-mRNA sequences with polyribosomes and transcription of histone-mRNA sequences from chromatin parallel DNA replication. Taken together these results suggest that the regulation of histone-gene expression resides, at least in part, at the transcriptional level. Chromatin-reconstitution studies provide evidence that non-histone chromosomal proteins play a key role in activation of histone-gene transcription during the period of the cell cycle when DNA is replicated. Phosphate groups associated with the S-phase non-histone chromosomal proteins appear to be functionally involved in the control of histone-gene readout.
G S Stein, and
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L J Kleinsmith, and
R L Jansing, and
W D Park, and
J A Thomson
March 1980,
Sheng li ke xue jin zhan [Progress in physiology],
G S Stein, and
J L Stein, and
L J Kleinsmith, and
R L Jansing, and
W D Park, and
J A Thomson
March 1975,
Harefuah,
G S Stein, and
J L Stein, and
L J Kleinsmith, and
R L Jansing, and
W D Park, and
J A Thomson
January 1976,
The ... Symposium. Society for Developmental Biology. Symposium,
G S Stein, and
J L Stein, and
L J Kleinsmith, and
R L Jansing, and
W D Park, and
J A Thomson
October 1975,
European journal of biochemistry,
G S Stein, and
J L Stein, and
L J Kleinsmith, and
R L Jansing, and
W D Park, and
J A Thomson
June 1973,
Hoppe-Seyler's Zeitschrift fur physiologische Chemie,
G S Stein, and
J L Stein, and
L J Kleinsmith, and
R L Jansing, and
W D Park, and
J A Thomson
November 1971,
Experimental cell research,
G S Stein, and
J L Stein, and
L J Kleinsmith, and
R L Jansing, and
W D Park, and
J A Thomson
April 2003,
Current opinion in genetics & development,
G S Stein, and
J L Stein, and
L J Kleinsmith, and
R L Jansing, and
W D Park, and
J A Thomson
November 1984,
Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme,
G S Stein, and
J L Stein, and
L J Kleinsmith, and
R L Jansing, and
W D Park, and
J A Thomson
April 1979,
European journal of biochemistry,
G S Stein, and
J L Stein, and
L J Kleinsmith, and
R L Jansing, and
W D Park, and
J A Thomson
March 1976,
European journal of biochemistry,
