Biomaterial Database

[The alpha-methylglucoside transport in Escherichia coli K12 cells]. 1977

M V Shul'gina, and I Ia Kalacheb, and G I Burd

The transport of alpha-methylglucoside (MG) in the wild type cells of Escherichia coli K12 and the isogenic mutant strains, defective in the activity of phosphoenolpyruvate: sugar phosphotransferase system components was studied. It was shown that the enzyme IIB' in the absence of enzyme I and HPr is able to transport MG into the cells by a "facilitated" diffusion mechanism. Compounds which dissipate the energy of membrane protone potential such as NaN3, carbonylcyanide-m-chlorophenylhydrasone, dicyclohexylcarbodiimide, enhance the utilization of MG by the wild-type cells. However, the cells retaining intact enzyme IIB' but deficient in the phospho approximately HPr-generating system, were not sensitive to the action of poisons. The cells possessing the intact phospho HPr-generating system and inactive enzyme IIB' are also unaffected by the poisons. It seems that these results do not confirm the hypothesis of the direct delta mu H+ involvement in the regulation of transmembrane phosphorylation. The hypothesis is postulated that the energy metabolism inhibitors influence the phosphatase activity of factor III of the phosphotransferase system. The present data are well explained by this hypothesis.

UI	MeSH Term	Description	Entries
D007700	Kinetics	The rate dynamics in chemical or physical systems.
D008757	Methylglucosides		Methylglucopyranosides
D008759	Methylglycosides
D009154	Mutation	Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.	Mutations
D004926	Escherichia coli	A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.	Alkalescens-Dispar Group,Bacillus coli,Bacterium coli,Bacterium coli commune,Diffusely Adherent Escherichia coli,E coli,EAggEC,Enteroaggregative Escherichia coli,Enterococcus coli,Diffusely Adherent E. coli,Enteroaggregative E. coli,Enteroinvasive E. coli,Enteroinvasive Escherichia coli
D001692	Biological Transport	The movement of materials (including biochemical substances and drugs) through a biological system at the cellular level. The transport can be across cell membranes and epithelial layers. It also can occur within intracellular compartments and extracellular compartments.	Transport, Biological,Biologic Transport,Transport, Biologic
D001693	Biological Transport, Active	The movement of materials across cell membranes and epithelial layers against an electrochemical gradient, requiring the expenditure of metabolic energy.	Active Transport,Uphill Transport,Active Biological Transport,Biologic Transport, Active,Transport, Active Biological,Active Biologic Transport,Transport, Active,Transport, Active Biologic,Transport, Uphill
D013045	Species Specificity	The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.	Species Specificities,Specificities, Species,Specificity, Species