Although mycoplasmas are commonly isolated in the genitourinary and upper respiratory tracts, they are rarely isolated from other sites. We describe a case of a young woman with trauma to her pelvis and genitourinary tract who had a thigh hematoma infected with Mycoplasma hominis. The patient had persistent systemic symptoms that resolved with appropriate antibiotics and surgical drainage. This case as well as other cases of Mycoplasma-infected hematomas point out the need to consider mycoplasma infection in patients with persistent fever unresponsive to conventional antibiotic therapy.