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De Novo Biosynthesis of Polydatin in Saccharomyces cerevisiae. 2021

Tian Liu, and Yuqian Liu, and Lan Li, and Xiaonan Liu, and Zhaokuan Guo, and Jian Cheng, and Xiaoxi Zhu, and Lina Lu, and Junlin Zhang, and Guanwei Fan, and Nengzhong Xie, and Jian Lu, and Huifeng Jiang
Life Science and Technology College, Guangxi University, Nanning, Guangxi 530004, China.

Polydatin, with better structural stability and biological activities than resveratrol, is mainly extracted from the traditional Chinese medicinal plant Polygonum cuspidatum. In this study, based on the transcriptome analysis of P. cuspidatum, we identified the key glycosyltransferase of resveratrol and achieved the biosynthesis of polydatin from glucose by incorporation with the resveratrol biosynthesis module, UDP-glucose supply module, and glycosyltransferase expression module. Through metabolic engineering and fermentation optimization, the production of polydatin reached 545 mg/L, and the dry cell weight was 27.83 mg/g DCW, which was about twice that of extracted from the P. cuspidatum root (11.404 mg/g DCW). Therefore, it is possible to replace the production mode of polydatin from plant extraction to microbial chassis in the future.

UI MeSH Term Description Entries
D005960 Glucosides A GLYCOSIDE that is derived from GLUCOSE. Glucoside
D012441 Saccharomyces cerevisiae A species of the genus SACCHAROMYCES, family Saccharomycetaceae, order Saccharomycetales, known as "baker's" or "brewer's" yeast. The dried form is used as a dietary supplement. Baker's Yeast,Brewer's Yeast,Candida robusta,S. cerevisiae,Saccharomyces capensis,Saccharomyces italicus,Saccharomyces oviformis,Saccharomyces uvarum var. melibiosus,Yeast, Baker's,Yeast, Brewer's,Baker Yeast,S cerevisiae,Baker's Yeasts,Yeast, Baker
D013267 Stilbenes Organic compounds that contain 1,2-diphenylethylene as a functional group. Stilbene,Stilbene Derivative,Stilbene Derivatives,Stilbenoid,Stilbenoids,Derivative, Stilbene,Derivatives, Stilbene
D045468 Fallopia japonica A plant species of the family POLYGONACEAE. Itadori tea is prepared from the root of this genus. Polygonum cuspidatum,Japanese Knotweed,Knotweed, Japanese,Fallopia japonicas,Japanese Knotweeds,Knotweeds, Japanese,Polygonum cuspidatums,cuspidatum, Polygonum,japonicas, Fallopia

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