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Chemically defined and xeno-free culture condition for human extended pluripotent stem cells. 2021
Bei Liu, and
Shi Chen, and
Yaxing Xu, and
Yulin Lyu, and
Jinlin Wang, and
Yuanyuan Du, and
Yongcheng Sun, and
Heming Liu, and
Haoying Zhou, and
Weifeng Lai, and
Anqi Xue, and
Ming Yin, and
Cheng Li, and
Yun Bai, and
Jun Xu, and
Hongkui Deng
Department of Cell Biology, School of Basic Medical Sciences, Peking University Stem Cell Research Center, State Key Laboratory of Natural and Biomimetic Drugs, Peking University Health Science Center and the MOE Key Laboratory of Cell Proliferation and Differentiation, College of Life Sciences, Peking-Tsinghua Center for Life Sciences, Peking University, Beijing, China.
Extended pluripotent stem (EPS) cells have shown great applicative potentials in generating synthetic embryos, directed differentiation and disease modeling. However, the lack of a xeno-free culture condition has significantly limited their applications. Here, we report a chemically defined and xeno-free culture system for culturing and deriving human EPS cells in vitro. Xeno-free human EPS cells can be long-term and genetically stably maintained in vitro, as well as preserve their embryonic and extraembryonic developmental potentials. Furthermore, the xeno-free culturing system also permits efficient derivation of human EPS cells from human fibroblast through reprogramming. Our study could have broad utility in future applications of human EPS cells in biomedicine.
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