Preconditioning exercise is a widely used strategy believed to enhance performance later the same day. The authors examined the influence of preconditioning exercises 6 hours prior to a time-to-exhaustion (TTE) test during treadmill running. Ten male competitive runners (age = 26 [3] y, height = 184 [8] cm, weight = 73 [9] kg, maximum oxygen consumption = 72 [7] mL·kg-1·min-1) did a preconditioning session of running (RUN) or resistance exercise (RES) or no morning exercise (NoEx) in a randomized order, separated by >72 hours. The RUN consisted of 15 minutes of low-intensity running and 4 × 15 seconds at race pace (21-24 km·h-1) on a treadmill; RES involved 5 minutes of low-intensity running and 2 × 3 repetitions of isokinetic 1-leg shallow squats with maximal mobilization. Following a 6-hour break, electrically evoked force (m. vastus medialis), countermovement jump, running economy, and a TTE of approximately 2 minutes were examined. Relative to NoEx, no difference was seen for RUN or RES in TTE (mean ± 95% CI: -1.3% ± 3.4% and -0.5% ± 6.0%) or running economy (0.2% ± 1.6% and 1.9% ± 2.7%; all Ps > .05). Jump height was not different for the RUN condition (1.0% ± 2.7%]) but tended to be higher in RES than in the NoEx condition (1.5% ± 1.6%, P = .07). The electrically evoked force tended to reveal low-frequency fatigue (reduced 20:50-Hz peak force ratio) only after RES compared to NoEx (-4.5% ± 4.6%, P = .06). The RUN or RES 6 hours prior to approximately 2 minutes of TTE running test did not improve performance in competitive runners.