We study the detection of continuous-variable entanglement, for which most of the existing methods designed so far require a full specification of the devices, and we present protocols for entanglement detection in a scenario where the measurement devices are completely uncharacterized. We first generalize, to the continuous variable regime, the seminal results by Buscemi [Phys. Rev. Lett. 108, 200401 (2012)PRLTAO0031-900710.1103/PhysRevLett.108.200401] and Branciard et al. [Phys. Rev. Lett. 110, 060405 (2013)PRLTAO0031-900710.1103/PhysRevLett.110.060405], showing that all entangled states can be detected in this scenario. Most importantly, we then describe a practical protocol that allows for the measurement-device-independent certification of entanglement of all two-mode entangled Gaussian states. This protocol is feasible with current technology as it makes use only of standard optical setups such as coherent states and homodyne measurements.