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We examined the effects of 18 h of incubation of Chinese hamster ovary (CHO K1) cells with cycloheximide, hydroxyurea, and aphidicolin. Treatment of cells with cycloheximide alone at a concentration adequate to inhibit DNA synthesis to less than 10% of control was significantly less cytotoxic and clastogenic than treatment with hydroxyurea or aphidicolin, did not induce unbalanced cellular growth, and had no effect on the frequency of resistant cells in methotrexate selections compared with control cells. When combined with hydroxyurea or aphidicolin and compared with the effects of either drug alone, cycloheximide blocked the induction of unbalanced growth during drug treatment, reduced the frequency of chromosomal aberrations in recovering cell populations, and decreased cell killing. In addition, the increased frequency of methotrexate-resistant cells observed after treatment with hydroxyurea or aphidicolin was eliminated when cycloheximide was present during drug treatment.
S W Sherwood, and
R I Schumacher, and
R T Schimke
July 1971,
Experimental cell research,
S W Sherwood, and
R I Schumacher, and
R T Schimke
July 1995,
Biochemical pharmacology,
S W Sherwood, and
R I Schumacher, and
R T Schimke
October 1981,
Cancer research,
S W Sherwood, and
R I Schumacher, and
R T Schimke
September 1979,
Human genetics,
S W Sherwood, and
R I Schumacher, and
R T Schimke
November 1976,
In vitro,
S W Sherwood, and
R I Schumacher, and
R T Schimke
May 1991,
Biochemical and biophysical research communications,
S W Sherwood, and
R I Schumacher, and
R T Schimke
November 1990,
Microbial pathogenesis,
S W Sherwood, and
R I Schumacher, and
R T Schimke
March 1975,
Mutation research,
S W Sherwood, and
R I Schumacher, and
R T Schimke
August 1987,
International journal of radiation biology and related studies in physics, chemistry, and medicine,
S W Sherwood, and
R I Schumacher, and
R T Schimke
October 1975,
Mutation research,
