Mechanical Permeabilization as a New Method for Assessment of Mitochondrial Function in Insect Tissues. 2021

Alessandro Gaviraghi, and Yan Aveiro, and Stephanie S Carvalho, and Rodiesley S Rosa, and Matheus P Oliveira, and Marcus F Oliveira
Federal University of Rio de Janeiro, Institute of Medical Biochemistry Leopoldo de Meis, Rio De Janeiro, RJ, Brazil.

Respirometry analysis is an effective technique to assess mitochondrial physiology. Insects are valuable biochemical models to understand metabolism and human diseases. Insect flight muscle and brain have been extensively used to explore mitochondrial function due to dissection feasibility and the low sample effort to allow oxygen consumption measurements. However, adequate plasma membrane permeabilization is required for substrates/modulators to reach mitochondria. Here, we describe a new method for study of mitochondrial physiology in insect tissues based on mechanical permeabilization as a fast and reliable method that do not require the use of detergents for chemical permeabilization of plasma membrane, while preserves mitochondrial integrity.

UI MeSH Term Description Entries
D008928 Mitochondria Semiautonomous, self-reproducing organelles that occur in the cytoplasm of all cells of most, but not all, eukaryotes. Each mitochondrion is surrounded by a double limiting membrane. The inner membrane is highly invaginated, and its projections are called cristae. Mitochondria are the sites of the reactions of oxidative phosphorylation, which result in the formation of ATP. They contain distinctive RIBOSOMES, transfer RNAs (RNA, TRANSFER); AMINO ACYL T RNA SYNTHETASES; and elongation and termination factors. Mitochondria depend upon genes within the nucleus of the cells in which they reside for many essential messenger RNAs (RNA, MESSENGER). Mitochondria are believed to have arisen from aerobic bacteria that established a symbiotic relationship with primitive protoeukaryotes. (King & Stansfield, A Dictionary of Genetics, 4th ed) Mitochondrial Contraction,Mitochondrion,Contraction, Mitochondrial,Contractions, Mitochondrial,Mitochondrial Contractions
D008931 Mitochondria, Muscle Mitochondria of skeletal and smooth muscle. It does not include myocardial mitochondria for which MITOCHONDRIA, HEART is available. Sarcosomes,Mitochondrion, Muscle,Muscle Mitochondria,Muscle Mitochondrion,Sarcosome
D010101 Oxygen Consumption The rate at which oxygen is used by a tissue; microliters of oxygen STPD used per milligram of tissue per hour; the rate at which oxygen enters the blood from alveolar gas, equal in the steady state to the consumption of oxygen by tissue metabolism throughout the body. (Stedman, 25th ed, p346) Consumption, Oxygen,Consumptions, Oxygen,Oxygen Consumptions
D010539 Permeability Property of membranes and other structures to permit passage of light, heat, gases, liquids, metabolites, and mineral ions. Permeabilities
D004330 Drosophila A genus of small, two-winged flies containing approximately 900 described species. These organisms are the most extensively studied of all genera from the standpoint of genetics and cytology. Fruit Fly, Drosophila,Drosophila Fruit Flies,Drosophila Fruit Fly,Drosophilas,Flies, Drosophila Fruit,Fly, Drosophila Fruit,Fruit Flies, Drosophila
D000330 Aedes A genus of mosquitoes (CULICIDAE) frequently found in tropical and subtropical regions. YELLOW FEVER and DENGUE are two of the diseases that can be transmitted by species of this genus. Aede
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D019069 Cell Respiration The metabolic process of all living cells (animal and plant) in which oxygen is used to provide a source of energy for the cell. Cellular Respiration,Respiration, Cell,Cell Respirations,Cellular Respirations,Respiration, Cellular

Related Publications

Alessandro Gaviraghi, and Yan Aveiro, and Stephanie S Carvalho, and Rodiesley S Rosa, and Matheus P Oliveira, and Marcus F Oliveira
July 1997, Gene therapy,
Alessandro Gaviraghi, and Yan Aveiro, and Stephanie S Carvalho, and Rodiesley S Rosa, and Matheus P Oliveira, and Marcus F Oliveira
May 2007, Apoptosis : an international journal on programmed cell death,
Alessandro Gaviraghi, and Yan Aveiro, and Stephanie S Carvalho, and Rodiesley S Rosa, and Matheus P Oliveira, and Marcus F Oliveira
March 2008, Urology,
Alessandro Gaviraghi, and Yan Aveiro, and Stephanie S Carvalho, and Rodiesley S Rosa, and Matheus P Oliveira, and Marcus F Oliveira
August 2007, Biotechnology and bioengineering,
Alessandro Gaviraghi, and Yan Aveiro, and Stephanie S Carvalho, and Rodiesley S Rosa, and Matheus P Oliveira, and Marcus F Oliveira
December 2020, Analytical biochemistry,
Alessandro Gaviraghi, and Yan Aveiro, and Stephanie S Carvalho, and Rodiesley S Rosa, and Matheus P Oliveira, and Marcus F Oliveira
November 1983, Stain technology,
Alessandro Gaviraghi, and Yan Aveiro, and Stephanie S Carvalho, and Rodiesley S Rosa, and Matheus P Oliveira, and Marcus F Oliveira
May 1971, Journal of cell science,
Alessandro Gaviraghi, and Yan Aveiro, and Stephanie S Carvalho, and Rodiesley S Rosa, and Matheus P Oliveira, and Marcus F Oliveira
February 2021, The Laryngoscope,
Alessandro Gaviraghi, and Yan Aveiro, and Stephanie S Carvalho, and Rodiesley S Rosa, and Matheus P Oliveira, and Marcus F Oliveira
March 1996, Biological & pharmaceutical bulletin,
Alessandro Gaviraghi, and Yan Aveiro, and Stephanie S Carvalho, and Rodiesley S Rosa, and Matheus P Oliveira, and Marcus F Oliveira
December 2021, Free radical biology & medicine,
Copied contents to your clipboard!