There are numerous scientific data about the study of the prevalence of blood group antigens in the different donor population. Several studies showed that the profile of major blood group antigens is not similar in blood donors from different local areas. OBJECTIVE Our scientific goal was to study of the prevalence blood group antigens in the Georgian blood donor population. In the current study, we analyzed the 48 phenotypically combinations based on four major (ABO, Rh, Kell, and MN) blood groups. METHODS The blood of 1009 donors has been studied on RBC antigens. The sample were collected from the diagnostic laboratory of Medina Ltd Health Centre of Batumi. Blood typing of the sample has been carried out on the basis of the immunogenetics laboratory of Batumi Shota Rustaveli State University. The universal monoclone antibodies was used for identify minor blood group antigens. We used as forward as reverse grouping methods. For identification erythrocytes, blood group antigens also were used ID cards, such as ABO/D + Reverse Grouping. RESULTS 12 phenotypic combinations have been identified in each O, A, B, AB group of ABO system. Out of 48 theoretically possible phenotypic combinations, we can actually find 1,9 times less phenotypes and the real amount is 25 phenotypes. The remaining 23 phenotypic combinations have not been observed in the studied donors. These are: 1. O, Rh-K+ MM; 2. O, Rh-K- MN; 3. O, Rh-K- NN; 4. A, Rh-K+ MN; 5. A, Rh-K+ MM; 6. A, Rh-K+ NN; 7. A, Rh-K- MM; 8. A, Rh-K- NN; 9. B, Rh+K+ NN; 10. B, Rh-K+ MN; 11. B, Rh-K+ MM; 12. B, Rh-K+ NN; 13. B, Rh-K- MN; 14. B, Rh-K- MM; 15. B, Rh-K- NN; 16. AB, Rh+K+ MN; 17. AB, Rh+K+ NN; 18. AB, Rh+K- NN; 19. AB, Rh+K- MM; 20. AB, Rh-K+ MN; 21. AB, Rh-K+ MM; 22. AB, Rh-K+ NN; 23. B, Rh-K- NN. The value of χ2 in the case is equal to 3221,16. The P-Value is < .00001. The result is significant at P < .05. Out of 1009 studied donors 349 are carriers of phenotypic group A (II), while 19 donors carry AB (IV) group specification. This means that 36.23% of the studied donors have A antigen on the surface of erythrocyte membrane. The majority of them A1 subgroup. CONCLUSIONS As our research showed there is a quit high polymorphism of blood group phenotype combinations in Georgian blood donors in the example of one clinic. This kind of data is very important for the clinics' rational preparation of whole blood or blood components.