TSH-like producing cells have been studied using the peroxidase-antiperoxidase (PAP) immunohistochemical technique and rabbit anti-human thyrotropin (TSH) antiserum in the pars distalis of five species of amphibian anuran tadpoles at different stages of development as well as in 1-year-old postmetamorphic animals. This cell type has been identified and located in the pars distalis ventral zone of Bufo calamita showing no changes in its distribution pattern throughout development, and in Rana perezi where TSH-like cells were located in the central zone of the gland in premetamorphic stages, occupying the ventral zone at the end of metamorphosis. No immunoreaction has been observed in the other species studied (Hyla meridionalis, Alytes cisternasii, and Pelobates cultripes). Morphometry and stereology were used to evaluate the changes observed in pars distalis volume and TSH immunoreactive cells during development. Pars distalis volume increased during the larval growth period and decreased throughout the metamorphic climax. TSH volume density and cellular area showed different evolutions in the species studied. The correlation between these parameters suggests a greatly proliferative period followed by an increase in cellular size. The changes observed in TSH total volume as well as the physiological data found by other authors lead us to conclude that there are two phases in amphibian development: first a period of TSH storage (pre- and prometamorphosis) and second a period of TSH release at metamorphic climax.