Biomaterial Database

Investigation of serum creatine kinase variability after muscle-damaging exercise. 1988

P M Clarkson, and C Ebbeling

Department of Exercise Science, University of Massachusetts, Amherst 01003.

1. The present study examined if the presence of creatine kinase (CK) inhibitors might explain the large variability in blood levels of CK among subjects after exercise-induced muscle damage. 2. Twenty-four women performed an eccentric exercise with the forearm flexors and were then classified as no CK responders, low CK responders and high CK responders. High CK responders repeated the exercise two weeks later (bout two). 3. Sera from high CK responders were mixed with sera from no CK responders or low CK responders. Also, serum from high CK responders obtained after bout one was mixed with the same subject's serum from after bout two. 4. In all cases, the differences between the expected and observed CK activity for the mixes were within the expected variability for the assay. 5. Although CK inhibitors have been previously observed in sera from patients with muscle injury or disease, we were unable to demonstrate the presence of CK inhibitors in the sera from subjects who showed evidence of exercise-induced muscle damage.

UI	MeSH Term	Description	Entries
D009135	Muscular Diseases	Acquired, familial, and congenital disorders of SKELETAL MUSCLE and SMOOTH MUSCLE.	Muscle Disorders,Myopathies,Myopathic Conditions,Muscle Disorder,Muscular Disease,Myopathic Condition,Myopathy
D003402	Creatine Kinase	A transferase that catalyzes formation of PHOSPHOCREATINE from ATP + CREATINE. The reaction stores ATP energy as phosphocreatine. Three cytoplasmic ISOENZYMES have been identified in human tissues: the MM type from SKELETAL MUSCLE, the MB type from myocardial tissue and the BB type from nervous tissue as well as a mitochondrial isoenzyme. Macro-creatine kinase refers to creatine kinase complexed with other serum proteins.	Creatine Phosphokinase,ADP Phosphocreatine Phosphotransferase,ATP Creatine Phosphotransferase,Macro-Creatine Kinase,Creatine Phosphotransferase, ATP,Kinase, Creatine,Macro Creatine Kinase,Phosphocreatine Phosphotransferase, ADP,Phosphokinase, Creatine,Phosphotransferase, ADP Phosphocreatine,Phosphotransferase, ATP Creatine
D005082	Physical Exertion	Expenditure of energy during PHYSICAL ACTIVITY. Intensity of exertion may be measured by rate of OXYGEN CONSUMPTION; HEAT produced, or HEART RATE. Perceived exertion, a psychological measure of exertion, is included.	Physical Effort,Effort, Physical,Efforts, Physical,Exertion, Physical,Exertions, Physical,Physical Efforts,Physical Exertions
D005260	Female		Females
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man