-transparent.png){kind=logo}
We examined the effects of newly exploited amiloride analogs on protein phosphorylation and serotonin secretion in human platelets. 5-(N-methyl-N-isobutyl) amiloride (IBA) and, to a lesser extent, 5-(N-methyl-N-isopropyl) amiloride (IPA), highly specific inhibitors of Na+/H+-pump, induced the phosphorylation of 47K-dalton protein and myosin light chain (20K). The phosphorylation was inhibited by apyrase. On the other hand, 3', 4'-dichlorobenzamil (DCB) and 2', 4'-dimethylbenzamil (DMB), highly specific inhibitors of Na+/Ca2+-pump, and to a lesser extent amiloride analogs induced serotonin secretion. Apparently there was dissociation between the phosphorylation and the serotonin release induced by the analogs.
K Yoshida, and
E J Cragoe
January 1996,
Platelets,
K Yoshida, and
E J Cragoe
June 1983,
Thrombosis research,
K Yoshida, and
E J Cragoe
December 1979,
Cell,
K Yoshida, and
E J Cragoe
June 1984,
FEBS letters,
K Yoshida, and
E J Cragoe
September 1998,
European journal of pharmacology,
K Yoshida, and
E J Cragoe
January 1955,
Journal of applied physiology,
K Yoshida, and
E J Cragoe
October 1973,
The Journal of laboratory and clinical medicine,
K Yoshida, and
E J Cragoe
April 1967,
Nature,
K Yoshida, and
E J Cragoe
July 1987,
Kidney international,
K Yoshida, and
E J Cragoe
October 1975,
The Journal of clinical investigation,
