-transparent.png){kind=logo}
Two-dimensional polyacrylamide gel electrophoresis has been used to detect somatic cell gene mutations altering protein structure, following ethylnitrosourea treatment of cultured human lymphoblastoid cells. A total of 267 polypeptides encoded by 263 loci were scored in a series of 1143 lymphoblastoid clones. Sixty-five electrophoretic mutants were detected at a total of 49 loci. Sixteen of the 65 mutations were phenotypically repeat mutations, occurring at 11 loci. Furthermore, structural mutations occurred more frequently at loci known to be polymorphic. These results provide evidence that the mutations that are detectable at the protein level by two-dimensional polyacrylamide gel electrophoresis do not occur at random and that their frequency is greater among polymorphic loci.
S M Hanash, and
M Boehnke, and
E H Chu, and
J V Neel, and
R D Kuick
February 1983,
Theoretical population biology,
S M Hanash, and
M Boehnke, and
E H Chu, and
J V Neel, and
R D Kuick
November 1983,
Life sciences,
S M Hanash, and
M Boehnke, and
E H Chu, and
J V Neel, and
R D Kuick
June 1972,
Gan,
S M Hanash, and
M Boehnke, and
E H Chu, and
J V Neel, and
R D Kuick
August 1983,
Science (New York, N.Y.),
S M Hanash, and
M Boehnke, and
E H Chu, and
J V Neel, and
R D Kuick
October 1981,
International journal of cancer,
S M Hanash, and
M Boehnke, and
E H Chu, and
J V Neel, and
R D Kuick
November 1976,
Journal of immunology (Baltimore, Md. : 1950),
S M Hanash, and
M Boehnke, and
E H Chu, and
J V Neel, and
R D Kuick
January 1984,
Cancer research,
S M Hanash, and
M Boehnke, and
E H Chu, and
J V Neel, and
R D Kuick
February 1976,
Human genetics,
S M Hanash, and
M Boehnke, and
E H Chu, and
J V Neel, and
R D Kuick
January 1976,
American journal of human genetics,
S M Hanash, and
M Boehnke, and
E H Chu, and
J V Neel, and
R D Kuick
January 1977,
Cytobios,
