BACKGROUND Nurses are on the front line of the healthcare system and should, therefore, have the evidence-based knowledge to manage breastfeeding. OBJECTIVE The objective of this study was to assess the attitudes and knowledge about human lactation among a group of nursing students. METHODS An anonymous online survey was sent to all College of Nursing students at a local university in Kentucky. RESULTS Nursing students participating in the survey favored breastfeeding over formula-feeding for infants. Lack of knowledge and various misconceptions about breastfeeding were reported. Whether a nursing student has or has not completed a class about human lactation during the nursing program, and has had or not children, significantly influences their attitudes and knowledge towards breastfeeding. CONCLUSIONS Future studies should focus on identifying if nursing faculty members and nursing students recognize human lactation as a value and central knowledge for nurses.