Costunolide Induces Apoptosis via the Reactive Oxygen Species and Protein Kinase B Pathway in Oral Cancer Cells. 2021

Hai Huang, and Jun-Koo Yi, and Su-Geun Lim, and Sijun Park, and Haibo Zhang, and Eungyung Kim, and Soyoung Jang, and Mee-Hyun Lee, and Kangdong Liu, and Ki-Rim Kim, and Eun-Kyong Kim, and Youngkyun Lee, and Sung-Hyun Kim, and Zae-Young Ryoo, and Myoung Ok Kim
Department of Animal Science and Biotechnology, ITRD, Kyungpook National University, Sangju 37224, Korea.

Oral cancer (OC) has been attracted research attention in recent years as result of its high morbidity and mortality. Costunolide (CTD) possesses potential anticancer and bioactive abilities that have been confirmed in several types of cancers. However, its effects on oral cancer remain unclear. This study investigated the potential anticancer ability and underlying mechanisms of CTD in OC in vivo and in vitro. Cell viability and anchorage-independent colony formation assays were performed to examine the antigrowth effects of CTD on OC cells; assessments for migration and invasion of OC cells were conducted by transwell; Cell cycle and apoptosis were investigated by flow cytometry and verified by immunoblotting. The results revealed that CTD suppressed the proliferation, migration and invasion of oral cancer cells effectively and induced cell cycle arrest and apoptosis; regarding the mechanism, CTD bound to AKT directly by binding assay and repressed AKT activities through kinase assay, which thereby downregulating the downstream of AKT. Furthermore, CTD remarkably promotes the generation of reactive oxygen species by flow cytometry assay, leading to cell apoptosis. Notably, CTD strongly suppresses cell-derived xenograft OC tumor growth in an in vivo mouse model. In conclusion, our results suggested that costunolide might prevent progression of OC and promise to be a novel AKT inhibitor.

UI MeSH Term Description Entries
D008819 Mice, Nude Mutant mice homozygous for the recessive gene "nude" which fail to develop a thymus. They are useful in tumor studies and studies on immune responses. Athymic Mice,Mice, Athymic,Nude Mice,Mouse, Athymic,Mouse, Nude,Athymic Mouse,Nude Mouse
D009062 Mouth Neoplasms Tumors or cancer of the MOUTH. Cancer of Mouth,Mouth Cancer,Oral Cancer,Oral Neoplasms,Cancer of the Mouth,Neoplasms, Mouth,Neoplasms, Oral,Cancer, Mouth,Cancer, Oral,Cancers, Mouth,Cancers, Oral,Mouth Cancers,Mouth Neoplasm,Neoplasm, Mouth,Neoplasm, Oral,Oral Cancers,Oral Neoplasm
D002453 Cell Cycle The complex series of phenomena, occurring between the end of one CELL DIVISION and the end of the next, by which cellular material is duplicated and then divided between two daughter cells. The cell cycle includes INTERPHASE, which includes G0 PHASE; G1 PHASE; S PHASE; and G2 PHASE, and CELL DIVISION PHASE. Cell Division Cycle,Cell Cycles,Cell Division Cycles,Cycle, Cell,Cycle, Cell Division,Cycles, Cell,Cycles, Cell Division,Division Cycle, Cell,Division Cycles, Cell
D002465 Cell Movement The movement of cells from one location to another. Distinguish from CYTOKINESIS which is the process of dividing the CYTOPLASM of a cell. Cell Migration,Locomotion, Cell,Migration, Cell,Motility, Cell,Movement, Cell,Cell Locomotion,Cell Motility,Cell Movements,Movements, Cell
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D000972 Antineoplastic Agents, Phytogenic Agents obtained from higher plants that have demonstrable cytostatic or antineoplastic activity. Antineoplastics, Botanical,Antineoplastics, Phytogenic,Agents, Phytogenic Antineoplastic,Botanical Antineoplastics,Phytogenic Antineoplastic Agents,Phytogenic Antineoplastics
D012717 Sesquiterpenes Fifteen-carbon compounds formed from three isoprenoid units with general formula C15H24. Farnesanes,Farnesene,Farnesenes,Sesquiterpene,Sesquiterpene Derivatives,Sesquiterpenoid,Sesquiterpenoids,Derivatives, Sesquiterpene
D014407 Tumor Cells, Cultured Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely. Cultured Tumor Cells,Neoplastic Cells, Cultured,Cultured Neoplastic Cells,Cell, Cultured Neoplastic,Cell, Cultured Tumor,Cells, Cultured Neoplastic,Cells, Cultured Tumor,Cultured Neoplastic Cell,Cultured Tumor Cell,Neoplastic Cell, Cultured,Tumor Cell, Cultured
D015398 Signal Transduction The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway. Cell Signaling,Receptor-Mediated Signal Transduction,Signal Pathways,Receptor Mediated Signal Transduction,Signal Transduction Pathways,Signal Transduction Systems,Pathway, Signal,Pathway, Signal Transduction,Pathways, Signal,Pathways, Signal Transduction,Receptor-Mediated Signal Transductions,Signal Pathway,Signal Transduction Pathway,Signal Transduction System,Signal Transduction, Receptor-Mediated,Signal Transductions,Signal Transductions, Receptor-Mediated,System, Signal Transduction,Systems, Signal Transduction,Transduction, Signal,Transductions, Signal

Related Publications

Hai Huang, and Jun-Koo Yi, and Su-Geun Lim, and Sijun Park, and Haibo Zhang, and Eungyung Kim, and Soyoung Jang, and Mee-Hyun Lee, and Kangdong Liu, and Ki-Rim Kim, and Eun-Kyong Kim, and Youngkyun Lee, and Sung-Hyun Kim, and Zae-Young Ryoo, and Myoung Ok Kim
December 2011, Gynecologic oncology,
Hai Huang, and Jun-Koo Yi, and Su-Geun Lim, and Sijun Park, and Haibo Zhang, and Eungyung Kim, and Soyoung Jang, and Mee-Hyun Lee, and Kangdong Liu, and Ki-Rim Kim, and Eun-Kyong Kim, and Youngkyun Lee, and Sung-Hyun Kim, and Zae-Young Ryoo, and Myoung Ok Kim
March 2017, Molecular medicine reports,
Hai Huang, and Jun-Koo Yi, and Su-Geun Lim, and Sijun Park, and Haibo Zhang, and Eungyung Kim, and Soyoung Jang, and Mee-Hyun Lee, and Kangdong Liu, and Ki-Rim Kim, and Eun-Kyong Kim, and Youngkyun Lee, and Sung-Hyun Kim, and Zae-Young Ryoo, and Myoung Ok Kim
January 2021, Frontiers in cell and developmental biology,
Hai Huang, and Jun-Koo Yi, and Su-Geun Lim, and Sijun Park, and Haibo Zhang, and Eungyung Kim, and Soyoung Jang, and Mee-Hyun Lee, and Kangdong Liu, and Ki-Rim Kim, and Eun-Kyong Kim, and Youngkyun Lee, and Sung-Hyun Kim, and Zae-Young Ryoo, and Myoung Ok Kim
October 2019, Cancers,
Hai Huang, and Jun-Koo Yi, and Su-Geun Lim, and Sijun Park, and Haibo Zhang, and Eungyung Kim, and Soyoung Jang, and Mee-Hyun Lee, and Kangdong Liu, and Ki-Rim Kim, and Eun-Kyong Kim, and Youngkyun Lee, and Sung-Hyun Kim, and Zae-Young Ryoo, and Myoung Ok Kim
December 2017, Oncotarget,
Hai Huang, and Jun-Koo Yi, and Su-Geun Lim, and Sijun Park, and Haibo Zhang, and Eungyung Kim, and Soyoung Jang, and Mee-Hyun Lee, and Kangdong Liu, and Ki-Rim Kim, and Eun-Kyong Kim, and Youngkyun Lee, and Sung-Hyun Kim, and Zae-Young Ryoo, and Myoung Ok Kim
January 2016, Nutrition and cancer,
Hai Huang, and Jun-Koo Yi, and Su-Geun Lim, and Sijun Park, and Haibo Zhang, and Eungyung Kim, and Soyoung Jang, and Mee-Hyun Lee, and Kangdong Liu, and Ki-Rim Kim, and Eun-Kyong Kim, and Youngkyun Lee, and Sung-Hyun Kim, and Zae-Young Ryoo, and Myoung Ok Kim
October 2015, Digestive diseases and sciences,
Hai Huang, and Jun-Koo Yi, and Su-Geun Lim, and Sijun Park, and Haibo Zhang, and Eungyung Kim, and Soyoung Jang, and Mee-Hyun Lee, and Kangdong Liu, and Ki-Rim Kim, and Eun-Kyong Kim, and Youngkyun Lee, and Sung-Hyun Kim, and Zae-Young Ryoo, and Myoung Ok Kim
March 2015, Environmental toxicology and pharmacology,
Hai Huang, and Jun-Koo Yi, and Su-Geun Lim, and Sijun Park, and Haibo Zhang, and Eungyung Kim, and Soyoung Jang, and Mee-Hyun Lee, and Kangdong Liu, and Ki-Rim Kim, and Eun-Kyong Kim, and Youngkyun Lee, and Sung-Hyun Kim, and Zae-Young Ryoo, and Myoung Ok Kim
February 2023, Phytotherapy research : PTR,
Hai Huang, and Jun-Koo Yi, and Su-Geun Lim, and Sijun Park, and Haibo Zhang, and Eungyung Kim, and Soyoung Jang, and Mee-Hyun Lee, and Kangdong Liu, and Ki-Rim Kim, and Eun-Kyong Kim, and Youngkyun Lee, and Sung-Hyun Kim, and Zae-Young Ryoo, and Myoung Ok Kim
January 2010, Biological & pharmaceutical bulletin,
Copied contents to your clipboard!