Development of a TaqMan-based real-time assay for the specific detection of canine astrovirus. 2021

Yongxia Wang, and Yongqiu Cui, and Xinxin Tong, and Yang Pan, and Xu Guo, and Fazhi Xu, and Yongdong Li, and Yong Wang
College of Animal Science and Technology, Anhui Agricultural University, Hefei, 230036, PR China.

In this study, a TaqMan-based real-time polymerase chain reaction (PCR) method to detect canine astrovirus in clinical samples was developed. Primers and probes were designed to target conserved regions of the complete viral genome sequence. The results showed that the proposed method can detect a minimum of 101 copy numbers. No cross-reactivity with other canine and feline viruses was observed. The coefficient of variation was <5%. Evaluation of the clinical samples showed that quantitative PCR had a 5.26 % higher positive detection rate than conventional PCR. These results indicate that the method developed in this study is highly reliable and suitable for veterinary clinical diagnosis and epidemiological investigations.

UI MeSH Term Description Entries
D002371 Cat Diseases Diseases of the domestic cat (Felis catus or F. domesticus). This term does not include diseases of the so-called big cats such as CHEETAHS; LIONS; tigers, cougars, panthers, leopards, and other Felidae for which the heading CARNIVORA is used. Feline Diseases,Cat Disease,Disease, Cat,Disease, Feline,Diseases, Cat,Diseases, Feline,Feline Disease
D002415 Cats The domestic cat, Felis catus, of the carnivore family FELIDAE, comprising over 30 different breeds. The domestic cat is descended primarily from the wild cat of Africa and extreme southwestern Asia. Though probably present in towns in Palestine as long ago as 7000 years, actual domestication occurred in Egypt about 4000 years ago. (From Walker's Mammals of the World, 6th ed, p801) Felis catus,Felis domesticus,Domestic Cats,Felis domestica,Felis sylvestris catus,Cat,Cat, Domestic,Cats, Domestic,Domestic Cat
D004283 Dog Diseases Diseases of the domestic dog (Canis familiaris). This term does not include diseases of wild dogs, WOLVES; FOXES; and other Canidae for which the heading CARNIVORA is used. Canine Diseases,Canine Disease,Disease, Canine,Disease, Dog,Diseases, Canine,Diseases, Dog,Dog Disease
D004285 Dogs The domestic dog, Canis familiaris, comprising about 400 breeds, of the carnivore family CANIDAE. They are worldwide in distribution and live in association with people. (Walker's Mammals of the World, 5th ed, p1065) Canis familiaris,Dog
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D001257 Mamastrovirus A genus of small, circular RNA viruses in the family ASTROVIRIDAE. They cause GASTROENTERITIS and are found in the stools of several vertebrates including humans. Transmission is by the fecal-oral route and there are at least eight human serotypes. The type species is Human astrovirus. Human astrovirus,Human astroviruses,Mamastroviruses,astroviruses, Human
D012680 Sensitivity and Specificity Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed) Specificity,Sensitivity,Specificity and Sensitivity
D060888 Real-Time Polymerase Chain Reaction Methods used for detecting the amplified DNA products from the polymerase chain reaction as they accumulate instead of at the end of the reaction. Kinetic Polymerase Chain Reaction,Quantitative Real-Time PCR,Quantitative Real-Time Polymerase Chain Reaction,Real-Time PCR,PCR, Quantitative Real-Time,PCR, Real-Time,PCRs, Quantitative Real-Time,PCRs, Real-Time,Quantitative Real Time PCR,Quantitative Real Time Polymerase Chain Reaction,Quantitative Real-Time PCRs,Real Time PCR,Real Time Polymerase Chain Reaction,Real-Time PCR, Quantitative,Real-Time PCRs,Real-Time PCRs, Quantitative
D017931 DNA Primers Short sequences (generally about 10 base pairs) of DNA that are complementary to sequences of messenger RNA and allow reverse transcriptases to start copying the adjacent sequences of mRNA. Primers are used extensively in genetic and molecular biology techniques. DNA Primer,Oligodeoxyribonucleotide Primer,Oligodeoxyribonucleotide Primers,Oligonucleotide Primer,Oligonucleotide Primers,Primer, DNA,Primer, Oligodeoxyribonucleotide,Primer, Oligonucleotide,Primers, DNA,Primers, Oligodeoxyribonucleotide,Primers, Oligonucleotide

Related Publications

Yongxia Wang, and Yongqiu Cui, and Xinxin Tong, and Yang Pan, and Xu Guo, and Fazhi Xu, and Yongdong Li, and Yong Wang
June 2021, Molecular and cellular probes,
Yongxia Wang, and Yongqiu Cui, and Xinxin Tong, and Yang Pan, and Xu Guo, and Fazhi Xu, and Yongdong Li, and Yong Wang
October 2017, Journal of virological methods,
Yongxia Wang, and Yongqiu Cui, and Xinxin Tong, and Yang Pan, and Xu Guo, and Fazhi Xu, and Yongdong Li, and Yong Wang
January 2022, Frontiers in veterinary science,
Yongxia Wang, and Yongqiu Cui, and Xinxin Tong, and Yang Pan, and Xu Guo, and Fazhi Xu, and Yongdong Li, and Yong Wang
November 2016, Journal of virological methods,
Yongxia Wang, and Yongqiu Cui, and Xinxin Tong, and Yang Pan, and Xu Guo, and Fazhi Xu, and Yongdong Li, and Yong Wang
December 2019, Microbial pathogenesis,
Yongxia Wang, and Yongqiu Cui, and Xinxin Tong, and Yang Pan, and Xu Guo, and Fazhi Xu, and Yongdong Li, and Yong Wang
August 2022, Journal of virological methods,
Yongxia Wang, and Yongqiu Cui, and Xinxin Tong, and Yang Pan, and Xu Guo, and Fazhi Xu, and Yongdong Li, and Yong Wang
June 2017, Avian pathology : journal of the W.V.P.A,
Yongxia Wang, and Yongqiu Cui, and Xinxin Tong, and Yang Pan, and Xu Guo, and Fazhi Xu, and Yongdong Li, and Yong Wang
June 2018, Molecular and cellular probes,
Yongxia Wang, and Yongqiu Cui, and Xinxin Tong, and Yang Pan, and Xu Guo, and Fazhi Xu, and Yongdong Li, and Yong Wang
August 2020, BMC veterinary research,
Yongxia Wang, and Yongqiu Cui, and Xinxin Tong, and Yang Pan, and Xu Guo, and Fazhi Xu, and Yongdong Li, and Yong Wang
January 2015, Medycyna doswiadczalna i mikrobiologia,
Copied contents to your clipboard!