Biomaterial Database

Nucleotide sequence of cDNA encoding the small subunit of ribulose-1,5-bisphosphate carboxylase from maize. 1987

M Matsuoka, and Y Kano-Murakami, and Y Tanaka, and Y Ozeki, and N Yamamoto

National Institute of Agrobiological Resources, Tsukuba Science City, Ibaraki.

We have cloned a full length cDNA for the small subunit of ribulose-1,5-bisphosphate carboxylase from C4 monocot maize, determined the complete nucleotide sequence of this cDNA and deduced its amino acid sequence. The cDNA insert included 513 bp of the coding region, and 65 and 252 nucleotides of the 5' and 3' untranslated regions, respectively. The transit and mature peptides have, respectively, 47 and 123 amino acids. Comparison with the small subunit genes from other plants revealed that the maize small subunit is similar to the wheat one, there being 73% homology between the transit peptides and 64% between the mature proteins. This indicates that there is no noteworthy difference between the C3 and C4 small subunit structures. Extreme codon bias was observed for this gene, and similar codon preferences are observed for other proteins highly expressed in maize leaf, light harvesting chlorophyll binding protein and phosphoenolpyruvate carboxylase. The results indicate that preferential codon usage for highly expressed genes occurs in maize leaf.

UI	MeSH Term	Description	Entries
D008969	Molecular Sequence Data	Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.	Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D003062	Codon	A set of three nucleotides in a protein coding sequence that specifies individual amino acids or a termination signal (CODON, TERMINATOR). Most codons are universal, but some organisms do not produce the transfer RNAs (RNA, TRANSFER) complementary to all codons. These codons are referred to as unassigned codons (CODONS, NONSENSE).	Codon, Sense,Sense Codon,Codons,Codons, Sense,Sense Codons
D003313	Zea mays	A plant species of the family POACEAE. It is a tall grass grown for its EDIBLE GRAIN, corn, used as food and animal FODDER.	Corn,Indian Corn,Maize,Teosinte,Zea,Corn, Indian
D004247	DNA	A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).	DNA, Double-Stranded,Deoxyribonucleic Acid,ds-DNA,DNA, Double Stranded,Double-Stranded DNA,ds DNA
D000595	Amino Acid Sequence	The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.	Protein Structure, Primary,Amino Acid Sequences,Sequence, Amino Acid,Sequences, Amino Acid,Primary Protein Structure,Primary Protein Structures,Protein Structures, Primary,Structure, Primary Protein,Structures, Primary Protein
D001483	Base Sequence	The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.	DNA Sequence,Nucleotide Sequence,RNA Sequence,DNA Sequences,Base Sequences,Nucleotide Sequences,RNA Sequences,Sequence, Base,Sequence, DNA,Sequence, Nucleotide,Sequence, RNA,Sequences, Base,Sequences, DNA,Sequences, Nucleotide,Sequences, RNA
D012273	Ribulose-Bisphosphate Carboxylase	A carboxy-lyase that plays a key role in photosynthetic carbon assimilation in the CALVIN-BENSON CYCLE by catalyzing the formation of 3-phosphoglycerate from ribulose 1,5-biphosphate and CARBON DIOXIDE. It can also utilize OXYGEN as a substrate to catalyze the synthesis of 2-phosphoglycolate and 3-phosphoglycerate in a process referred to as photorespiration.	Carboxydismutase,Ribulose Biphosphate Carboxylase-Oxygenase,Ribulose Diphosphate Carboxylase,Ribulosebiphosphate Carboxylase,Rubisco,1,5-Biphosphate Carboxylase-Oxygenase,Ribulose Biphosphate Carboxylase,Ribulose Bisphosphate Carboxylase,Ribulose-1,5-Biphosphate Carboxylase,Ribulose-1,5-Biphosphate Carboxylase-Oxygenase,Ribulose-1,5-Bisphosphate Carboxylase Small-Subunit,Ribulose-Bisphosphate Carboxylase Large Subunit,Ribulose-Bisphosphate Carboxylase Small Subunit,Rubisco Small Subunit,1,5 Biphosphate Carboxylase Oxygenase,Biphosphate Carboxylase-Oxygenase, Ribulose,Carboxylase Small-Subunit, Ribulose-1,5-Bisphosphate,Carboxylase, Ribulose Bisphosphate,Carboxylase, Ribulose Diphosphate,Carboxylase, Ribulose-1,5-Biphosphate,Carboxylase, Ribulose-Bisphosphate,Carboxylase, Ribulosebiphosphate,Carboxylase-Oxygenase, 1,5-Biphosphate,Carboxylase-Oxygenase, Ribulose Biphosphate,Carboxylase-Oxygenase, Ribulose-1,5-Biphosphate,Diphosphate Carboxylase, Ribulose,Ribulose 1,5 Biphosphate Carboxylase,Ribulose 1,5 Biphosphate Carboxylase Oxygenase,Ribulose 1,5 Bisphosphate Carboxylase Small Subunit,Ribulose Biphosphate Carboxylase Oxygenase,Ribulose Bisphosphate Carboxylase Large Subunit,Ribulose Bisphosphate Carboxylase Small Subunit,Small Subunit, Rubisco,Small-Subunit, Ribulose-1,5-Bisphosphate Carboxylase