Biomaterial Database

Leptospiral attachment to extracellular matrix of mouse fibroblast (L929) cells. 1987

T Ito, and R Yanagawa

Department of Hygiene and Microbiology, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Japan.

The attachment of leptospires to the extracellular matrix (ECM) remaining after mouse fibroblast (L929) cells on coverslips had been solubilized with Triton X-100 was examined. Each highly virulent line of Leptospira interrogans serovar copenhageni, canicola and pomona attached to ECM more effectively than intermediately virulent and avirulent lines of the same strains, suggesting a correlation between virulence and attachment to ECM. Inhibition of the attachment of highly virulent copenhageni to ECM was found in the presence of the homologous immunoglobulin G Fab fragment.

UI	MeSH Term	Description	Entries
D007074	Immunoglobulin G	The major immunoglobulin isotype class in normal human serum. There are several isotype subclasses of IgG, for example, IgG1, IgG2A, and IgG2B.	Gamma Globulin, 7S,IgG,IgG Antibody,Allerglobuline,IgG(T),IgG1,IgG2,IgG2A,IgG2B,IgG3,IgG4,Immunoglobulin GT,Polyglobin,7S Gamma Globulin,Antibody, IgG,GT, Immunoglobulin
D007140	Immunoglobulin Fab Fragments	Univalent antigen-binding fragments composed of one entire IMMUNOGLOBULIN LIGHT CHAIN and the amino terminal end of one of the IMMUNOGLOBULIN HEAVY CHAINS from the hinge region, linked to each other by disulfide bonds. Fab contains the IMMUNOGLOBULIN VARIABLE REGIONS, which are part of the antigen-binding site, and the first IMMUNOGLOBULIN CONSTANT REGIONS. This fragment can be obtained by digestion of immunoglobulins with the proteolytic enzyme PAPAIN.	Fab Fragment,Fab Fragments,Ig Fab Fragments,Immunoglobulins, Fab Fragment,Fab Immunoglobulin Fragments,Immunoglobulin Fab Fragment,Immunoglobulins, Fab,Fab Fragment Immunoglobulins,Fab Fragment, Immunoglobulin,Fab Fragments, Immunoglobulin,Fragment Immunoglobulins, Fab,Fragment, Fab,Immunoglobulin Fragments, Fab
D007921	Leptospira interrogans	A genus of question mark-shaped bacteria spirochetes which is found in fresh water that is contaminated by animal urine. It causes LEPTOSPIROSIS.	Leptospira icteroides,Spirochaeta icterogenes,Spirochaeta icterohaemorrhagiae,Spirochaeta interrogans,Spirochaeta nodosa
D002460	Cell Line	Established cell cultures that have the potential to propagate indefinitely.	Cell Lines,Line, Cell,Lines, Cell
D005109	Extracellular Matrix	A meshwork-like substance found within the extracellular space and in association with the basement membrane of the cell surface. It promotes cellular proliferation and provides a supporting structure to which cells or cell lysates in culture dishes adhere.	Matrix, Extracellular,Extracellular Matrices,Matrices, Extracellular
D005347	Fibroblasts	Connective tissue cells which secrete an extracellular matrix rich in collagen and other macromolecules.	Fibroblast
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D001422	Bacterial Adhesion	Physicochemical property of fimbriated (FIMBRIAE, BACTERIAL) and non-fimbriated bacteria of attaching to cells, tissue, and nonbiological surfaces. It is a factor in bacterial colonization and pathogenicity.	Adhesion, Bacterial,Adhesions, Bacterial,Bacterial Adhesions
D013696	Temperature	The property of objects that determines the direction of heat flow when they are placed in direct thermal contact. The temperature is the energy of microscopic motions (vibrational and translational) of the particles of atoms.	Temperatures
D014774	Virulence	The degree of pathogenicity within a group or species of microorganisms or viruses as indicated by case fatality rates and/or the ability of the organism to invade the tissues of the host. The pathogenic capacity of an organism is determined by its VIRULENCE FACTORS.	Pathogenicity

Related Publications

T Ito, and R Yanagawa

Leptospiral attachment to cultured cells.

December 1984, Zentralblatt fur Bakteriologie, Mikrobiologie, und Hygiene. Series A, Medical microbiology, infectious diseases, virology, parasitology,

T Ito, and R Yanagawa

Gliotoxin induces apoptosis in mouse L929 fibroblast cells.

June 1994, Biochemistry and molecular biology international,

T Ito, and R Yanagawa

Selection and characterization of interferon response mutants from mouse L929 fibroblast cells.

February 1988, The Journal of general virology,

T Ito, and R Yanagawa

Mechanism of fibroblast attachment to bone extracellular matrix: role of a 44 kilodalton bone phosphoprotein.

June 1987, Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research,

T Ito, and R Yanagawa

Attachment of neuroblastoma cells to extracellular matrix: correlation with metastatic capacity.

May 1989, The Journal of laboratory and clinical medicine,