Biomaterial Database

Molecularly Imprinted Polymer-Based Quartz Crystal Microbalance Sensor for the Clinical Detection of Insulin. 2021

Duygu Çimen, and Nilay Bereli, and Fatma Kartal, and Adil Denizli

Department of Chemistry, Hacettepe University, Ankara, Turkey.

In this study, we reported the design of a quartz crystal microbalance (QCM) sensors for selective insulin detection. In the first step, N-methacryloyl-(L) 3-histidine methyl ester (MAH) monomer was formed a complex with insulin. Then, 2-hydroxyethyl methacrylate and ethylene glycol dimethacrylate were mixed with MAH:insulin complex. Insulin-imprinted and non-imprinted QCM sensors were synthesized by ultraviolet polymerization for the insulin detection. Insulin-imprinted QCM sensors was characterized by the contact angle measurements, atomic force microscopy and ellipsometry. Limit of detection (LOD) was found as 0.00158 ng/mL for the insulin-imprinted QCM sensors. Selectivity of insulin-imprinted and non-imprinted QCM sensors was carried in the presence of glucagon and aprotinin. Insulin-imprinted QCM sensor for insulin detection was also examined in the artificial plasma.

UI	MeSH Term	Description	Entries
D007328	Insulin	A 51-amino acid pancreatic hormone that plays a major role in the regulation of glucose metabolism, directly by suppressing endogenous glucose production (GLYCOGENOLYSIS; GLUCONEOGENESIS) and indirectly by suppressing GLUCAGON secretion and LIPOLYSIS. Native insulin is a globular protein comprised of a zinc-coordinated hexamer. Each insulin monomer containing two chains, A (21 residues) and B (30 residues), linked by two disulfide bonds. Insulin is used as a drug to control insulin-dependent diabetes mellitus (DIABETES MELLITUS, TYPE 1).	Iletin,Insulin A Chain,Insulin B Chain,Insulin, Regular,Novolin,Sodium Insulin,Soluble Insulin,Chain, Insulin B,Insulin, Sodium,Insulin, Soluble,Regular Insulin
D011108	Polymers	Compounds formed by the joining of smaller, usually repeating, units linked by covalent bonds. These compounds often form large macromolecules (e.g., BIOPOLYMERS; PLASTICS).	Polymer
D011791	Quartz	Quartz (SiO2). A glassy or crystalline form of silicon dioxide. Many colored varieties are semiprecious stones. (From Grant & Hackh's Chemical Dictionary, 5th ed)
D000082582	Molecularly Imprinted Polymers	Synthetic polymeric matrices with artificial molecular recognition sites generated by MOLECULAR IMPRINTING TECHNIQUE. It can specifically bind target molecules (e.g., antibodies, enzymes or receptors).	Imprinted Polymer, Molecularly,Molecularly Imprinted Polymer,Polymer, Molecularly Imprinted
D015374	Biosensing Techniques	Any of a variety of procedures which use biomolecular probes to measure the presence or concentration of biological molecules, biological structures, microorganisms, etc., by translating a biochemical interaction at the probe surface into a quantifiable physical signal.	Bioprobes,Biosensors,Electrodes, Enzyme,Biosensing Technics,Bioprobe,Biosensing Technic,Biosensing Technique,Biosensor,Electrode, Enzyme,Enzyme Electrode,Enzyme Electrodes,Technic, Biosensing,Technics, Biosensing,Technique, Biosensing,Techniques, Biosensing
D054802	Molecular Imprinting	A methodology for chemically synthesizing polymer molds of specific molecules or recognition sites of specific molecules. Applications for MOLECULARLY IMPRINTED POLYMERS (MIPs) include separations, assays and biosensors, and catalysis.	Molecular Imprinting Technique,Imprinting, Molecular,Imprinting Technique, Molecular,Imprinting Techniques, Molecular,Molecular Imprinting Techniques,Technique, Molecular Imprinting,Techniques, Molecular Imprinting
D058525	Quartz Crystal Microbalance Techniques	The use of a quartz crystal microbalance for measuring weights and forces in the micro- to nanogram range. It is used to study the chemical and mechanical properties of thin layers, such as polymer coatings and lipid membranes; and interactions between molecues.	Electrochemical Quartz Crystal Impedance Analysis,Quartz Crystal Microbalance,Quartz Crystal Microbalance with Dissipation Monitoring,Microbalance, Quartz Crystal,Microbalances, Quartz Crystal,Quartz Crystal Microbalances