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In vitro recombination techniques were used to construct a new cloning vehicle, pBR322. This plasmid, derived from pBR313, is a relaxed replicating plasmid, does not produce and is sensitive to colicin E1, and carries resistance genes to the antibiotics ampicillin (Ap) and tetracycline (Tc). The antibiotic-resistant genes on pBR322 are not transposable. The vector pBR322 was constructed in order to have a plasmid with a single PstI site, located in the ampicillin-resistant gene (Apr), in addition to four unique restriction sites, EcoRI, HindIII, BamHI and SalI. Survival of Escherichia coli strain X1776 containing pBR313 and pBR322 as a function of thymine and diaminopimelic acid (DAP) starvation and sensitivity to bile salts was found to be equivalent to the non-plasmid containing strain. Conjugal transfer of these plasmids in bi- and triparental matings were significantly reduced or undetectable relative to the plasmid ColE1.
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M C Betlach, and
H L Heyneker, and
H W Boyer, and
J H Crosa, and
S Falkow
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H W Boyer, and
J H Crosa, and
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January 1982,
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F Bolivar, and
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H L Heyneker, and
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S Falkow
April 1981,
Nucleic acids research,
F Bolivar, and
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S Falkow
April 1984,
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F Bolivar, and
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P J Greene, and
M C Betlach, and
H L Heyneker, and
H W Boyer, and
J H Crosa, and
S Falkow
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Gene,
F Bolivar, and
R L Rodriguez, and
P J Greene, and
M C Betlach, and
H L Heyneker, and
H W Boyer, and
J H Crosa, and
S Falkow
June 1978,
Journal of bacteriology,
F Bolivar, and
R L Rodriguez, and
P J Greene, and
M C Betlach, and
H L Heyneker, and
H W Boyer, and
J H Crosa, and
S Falkow
January 1981,
Gene,
F Bolivar, and
R L Rodriguez, and
P J Greene, and
M C Betlach, and
H L Heyneker, and
H W Boyer, and
J H Crosa, and
S Falkow
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F Bolivar, and
R L Rodriguez, and
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H W Boyer, and
J H Crosa, and
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April 1989,
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