Hydrogel film composed of chitosan (CS) as raw material was prepared by free radical polymerization. Silicon was introduced into the hydrogel film in different ways (covalent/non-covalent) to improve the physical properties of the film, and β-acids were loaded to enhance the antibacterial activity of the film. Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and X-ray diffraction (XRD) analysis were used to characterize the structure of films. The mechanical results indicated that when nano-silica (0.3%) was introduced into film (containing 0.2% β-acids) by non-covalently bond, the tensile strength increased to 8.59 MPa. Meanwhile silicon (0.3%) entered the film by covalent bonding, the tensile strength increased to 7.99 MPa. The films loaded with β-acids had well ability to blocks ultraviolet rays and exhibited inhibitory effect on E. coli and S. aureus. In the PBS (37 °C, pH = 7.4) simulant solution, the release mechanism of most films to release the β-acids followed non-Fick diffusion (n > 0.5). It could be concluded that the prepared hydrogel films loading with β-acids had broad application prospects in food packaging material with antibacterial property and controlled release.