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The data of genetic mapping of the cholera toxin regulatory gene by conjugation mating of Vibrio cholerae eltor donor strain with V. cholerae classica recipients are presented. The close genetic linkage of tox locus to pur-63 is shown. The gene order asp - cys - nal - pur-61 - trp - his - pur-63 - tox - ile of the chromosomal region examined is established.
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