Development of an aerogenous Escherichia coli infection model in adult broiler breeders. 2021

Sofie Kromann, and Rikke Heidemann Olsen, and Anders Miki Bojesen, and Henrik Elvang Jensen, and Ida Thøfner
Department of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Ridebanevej 3, 1870, Frederiksberg C, Denmark. sok@sund.ku.dk.

Escherichia coli constitutes an immense challenge to the poultry industry due to its devastating effect on productivity, mortality, and carcass condemnations. To aid future studies on disease mechanisms and interventions, an aerogenous infection model was established in adult broiler breeders. Hens (n = 120) were randomly allocated into six groups receiving either aerosolised E. coli or vehicle, or intratracheal E. coli or vehicle. Replication of aerosol inoculation was performed on distinct days. Alternating euthanasia time points were predetermined in order to evaluate the progression of the disease. All animals were thoroughly necropsied, and bacteriological samples were collected as well as tissues for histopathology. Birds inoculated with E. coli exhibited clinical signs and developed characteristic gross and histopathological lesions of colibacillosis, including splenic fibrinoid necrosis, folliculitis, polyserositis and impaction of parabronchi with fibrinoheterophilic exudate and necrotic debris, as well as positive in situ localisation of intralesional E. coli by immunohistochemistry. This study presents a successful development of a discriminative colibacillosis model through aerosol inoculation of adult broiler breeders. Gross and histopathological lesions characteristic of colibacillosis were established in two independent experiments.

UI MeSH Term Description Entries
D009026 Mortality All deaths reported in a given population. CFR Case Fatality Rate,Crude Death Rate,Crude Mortality Rate,Death Rate,Age Specific Death Rate,Age-Specific Death Rate,Case Fatality Rate,Decline, Mortality,Determinants, Mortality,Differential Mortality,Excess Mortality,Mortality Decline,Mortality Determinants,Mortality Rate,Mortality, Differential,Mortality, Excess,Age-Specific Death Rates,Case Fatality Rates,Crude Death Rates,Crude Mortality Rates,Death Rate, Age-Specific,Death Rate, Crude,Death Rates,Determinant, Mortality,Differential Mortalities,Excess Mortalities,Mortalities,Mortality Declines,Mortality Determinant,Mortality Rate, Crude,Mortality Rates,Rate, Age-Specific Death,Rate, Case Fatality,Rate, Crude Death,Rate, Crude Mortality,Rate, Death,Rate, Mortality,Rates, Case Fatality
D011201 Poultry Diseases Diseases of birds which are raised as a source of meat or eggs for human consumption and are usually found in barnyards, hatcheries, etc. The concept is differentiated from BIRD DISEASES which is for diseases of birds not considered poultry and usually found in zoos, parks, and the wild. Disease, Poultry,Diseases, Poultry,Poultry Disease
D002645 Chickens Common name for the species Gallus gallus, the domestic fowl, in the family Phasianidae, order GALLIFORMES. It is descended from the red jungle fowl of SOUTHEAST ASIA. Gallus gallus,Gallus domesticus,Gallus gallus domesticus,Chicken
D004195 Disease Models, Animal Naturally-occurring or experimentally-induced animal diseases with pathological processes analogous to human diseases. Animal Disease Model,Animal Disease Models,Disease Model, Animal
D004926 Escherichia coli A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc. Alkalescens-Dispar Group,Bacillus coli,Bacterium coli,Bacterium coli commune,Diffusely Adherent Escherichia coli,E coli,EAggEC,Enteroaggregative Escherichia coli,Enterococcus coli,Diffusely Adherent E. coli,Enteroaggregative E. coli,Enteroinvasive E. coli,Enteroinvasive Escherichia coli
D004927 Escherichia coli Infections Infections with bacteria of the species ESCHERICHIA COLI. E coli Infections,E. coli Infection,Infections, E coli,Infections, Escherichia coli,E coli Infection,E. coli Infections,Escherichia coli Infection,Infection, E coli,Infection, E. coli,Infection, Escherichia coli
D000336 Aerosols Colloids with a gaseous dispersing phase and either liquid (fog) or solid (smoke) dispersed phase; used in fumigation or in inhalation therapy; may contain propellant agents. Aerosol
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D001706 Biopsy Removal and pathologic examination of specimens from the living body. Biopsies
D015373 Bacterial Typing Techniques Procedures for identifying types and strains of bacteria. The most frequently employed typing systems are BACTERIOPHAGE TYPING and SEROTYPING as well as bacteriocin typing and biotyping. Bacteriocin Typing,Biotyping, Bacterial,Typing, Bacterial,Bacterial Biotyping,Bacterial Typing,Bacterial Typing Technic,Bacterial Typing Technics,Bacterial Typing Technique,Technic, Bacterial Typing,Technics, Bacterial Typing,Technique, Bacterial Typing,Techniques, Bacterial Typing,Typing Technic, Bacterial,Typing Technics, Bacterial,Typing Technique, Bacterial,Typing Techniques, Bacterial,Typing, Bacteriocin

Related Publications

Sofie Kromann, and Rikke Heidemann Olsen, and Anders Miki Bojesen, and Henrik Elvang Jensen, and Ida Thøfner
October 2023, Pathogens (Basel, Switzerland),
Sofie Kromann, and Rikke Heidemann Olsen, and Anders Miki Bojesen, and Henrik Elvang Jensen, and Ida Thøfner
June 2017, Avian pathology : journal of the W.V.P.A,
Sofie Kromann, and Rikke Heidemann Olsen, and Anders Miki Bojesen, and Henrik Elvang Jensen, and Ida Thøfner
August 2017, Veterinary microbiology,
Sofie Kromann, and Rikke Heidemann Olsen, and Anders Miki Bojesen, and Henrik Elvang Jensen, and Ida Thøfner
January 2005, Comparative immunology, microbiology and infectious diseases,
Sofie Kromann, and Rikke Heidemann Olsen, and Anders Miki Bojesen, and Henrik Elvang Jensen, and Ida Thøfner
March 1991, Avian pathology : journal of the W.V.P.A,
Sofie Kromann, and Rikke Heidemann Olsen, and Anders Miki Bojesen, and Henrik Elvang Jensen, and Ida Thøfner
August 2005, Avian pathology : journal of the W.V.P.A,
Sofie Kromann, and Rikke Heidemann Olsen, and Anders Miki Bojesen, and Henrik Elvang Jensen, and Ida Thøfner
March 2013, Veterinary microbiology,
Sofie Kromann, and Rikke Heidemann Olsen, and Anders Miki Bojesen, and Henrik Elvang Jensen, and Ida Thøfner
June 2020, Acta veterinaria Scandinavica,
Sofie Kromann, and Rikke Heidemann Olsen, and Anders Miki Bojesen, and Henrik Elvang Jensen, and Ida Thøfner
July 2007, Poultry science,
Sofie Kromann, and Rikke Heidemann Olsen, and Anders Miki Bojesen, and Henrik Elvang Jensen, and Ida Thøfner
September 1988, The Veterinary record,
Copied contents to your clipboard!