BIOMAT Database Logo BIOMAT Database Logo

Antibiotic resistance in the pathogenic foodborne bacteria isolated from raw kebab and hamburger: phenotypic and genotypic study. 2021

Maryam Rajaei, and Mir-Hassan Moosavy, and Sahar Nouri Gharajalar, and Seyed Amin Khatibi
Department of Food Hygiene and Aquatic, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran.

In recent years, interest in the consumption of ready-to-eat (RTE) food products has been increased in many countries. However, RTE products particularly those prepared by meat may be potential vehicles of antibiotic-resistance foodborne pathogens. Considering kebab and hamburger are the most popular RTE meat products in Iran, this study aimed to investigate the prevalence and antimicrobial resistance of common foodborne pathogens (Escherichia coli, Salmonella spp., Staphylococcus aureus, and Listeria monocytogenes) in raw kebab and hamburger samples collected from fast-food centers and restaurants. Therefore, total bacterial count (TBC), as well as the prevalence rates and antibiogram patterns of foodborne pathogens in the samples were investigated. Also, the presence of antibiotic-resistance genes (blaSHV, blaTEM, blaZ, and mecA) was studied in the isolates by PCR. The mean value of TBC in raw kebab and hamburger samples was 6.72 ± 0.68 log CFU/g and 6.64 ± 0.66 log CFU/g, respectively. E. coli had the highest prevalence rate among the investigated pathogenic bacteria in kebab (70%) and hamburger samples (48%). Salmonella spp., L. monocytogenes, and S. aureus were also recovered from 58, 50, and 36% of kebab samples, respectively. The contamination of hamburger samples was detected to S. aureus (22%), L. monocytogenes (22%), and Salmonella spp. (10%). In the antimicrobial susceptibility tests, all isolates exhibited high rates of antibiotic resistance, particularly against amoxicillin, penicillin, and cefalexin (79.66-100%). The blaTEM was the most common resistant gene in the isolates of E. coli (52.54%) and Salmonella spp. (44.11%). Fourteen isolates (23.72%) of E. coli and 10 isolates (29.41%) of Salmonella spp. were positive for blaSHV. Also, 16 isolates (55.17%) of S. aureus and 10 isolates (27.27%) of L. monocytogenes were positive for mecA gene. The findings of this study showed that raw kebab and hamburger are potential carriers of antibiotic-resistance pathogenic bacteria, which can be a serious threat to public health.

UI MeSH Term Description Entries
D007492 Iran A country bordering the Gulf of Oman, the Persian Gulf, and the Caspian Sea, between Iraq and Pakistan. The capital is Tehran. Islamic Republic of Iran
D008460 Meat The edible portions of any animal used for food including cattle, swine, goats/sheep, poultry, fish, shellfish, and game. Meats
D005516 Food Microbiology The presence of bacteria, viruses, and fungi in food and food products. This term is not restricted to pathogenic organisms: the presence of various non-pathogenic bacteria and fungi in cheeses and wines, for example, is included in this concept. Microbiology, Food
D005798 Genes, Bacterial The functional hereditary units of BACTERIA. Bacterial Gene,Bacterial Genes,Gene, Bacterial
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D000900 Anti-Bacterial Agents Substances that inhibit the growth or reproduction of BACTERIA. Anti-Bacterial Agent,Anti-Bacterial Compound,Anti-Mycobacterial Agent,Antibacterial Agent,Antibiotics,Antimycobacterial Agent,Bacteriocidal Agent,Bacteriocide,Anti-Bacterial Compounds,Anti-Mycobacterial Agents,Antibacterial Agents,Antibiotic,Antimycobacterial Agents,Bacteriocidal Agents,Bacteriocides,Agent, Anti-Bacterial,Agent, Anti-Mycobacterial,Agent, Antibacterial,Agent, Antimycobacterial,Agent, Bacteriocidal,Agents, Anti-Bacterial,Agents, Anti-Mycobacterial,Agents, Antibacterial,Agents, Antimycobacterial,Agents, Bacteriocidal,Anti Bacterial Agent,Anti Bacterial Agents,Anti Bacterial Compound,Anti Bacterial Compounds,Anti Mycobacterial Agent,Anti Mycobacterial Agents,Compound, Anti-Bacterial,Compounds, Anti-Bacterial
D001419 Bacteria One of the three domains of life (the others being Eukarya and ARCHAEA), also called Eubacteria. They are unicellular prokaryotic microorganisms which generally possess rigid cell walls, multiply by cell division, and exhibit three principal forms: round or coccal, rodlike or bacillary, and spiral or spirochetal. Bacteria can be classified by their response to OXYGEN: aerobic, anaerobic, or facultatively anaerobic; by the mode by which they obtain their energy: chemotrophy (via chemical reaction) or PHOTOTROPHY (via light reaction); for chemotrophs by their source of chemical energy: CHEMOLITHOTROPHY (from inorganic compounds) or chemoorganotrophy (from organic compounds); and by their source for CARBON; NITROGEN; etc.; HETEROTROPHY (from organic sources) or AUTOTROPHY (from CARBON DIOXIDE). They can also be classified by whether or not they stain (based on the structure of their CELL WALLS) with CRYSTAL VIOLET dye: gram-negative or gram-positive. Eubacteria
D057140 Fast Foods Prepared food that is ready to eat or partially prepared food that has a final preparation time of a few minutes or less. Convenience Foods,Meals, Ready-To-Eat,Ready-Prepared Foods,Ready-To-Eat Meals,Convenience Food,Fast Food,Food, Convenience,Food, Fast,Food, Ready-Prepared,Foods, Convenience,Foods, Fast,Foods, Ready-Prepared,Meal, Ready-To-Eat,Meals, Ready To Eat,Ready Prepared Foods,Ready To Eat Meals,Ready-Prepared Food,Ready-To-Eat Meal
D024881 Drug Resistance, Bacterial The ability of bacteria to resist or to become tolerant to chemotherapeutic agents, antimicrobial agents, or antibiotics. This resistance may be acquired through gene mutation or foreign DNA in transmissible plasmids (R FACTORS). Antibiotic Resistance, Bacterial,Antibacterial Drug Resistance
D066256 Raw Foods Food prepared for consumption without exposure to heat. Uncooked Foods,Food, Raw,Food, Uncooked,Foods, Raw,Foods, Uncooked,Raw Food,Uncooked Food

Related Publications

Maryam Rajaei, and Mir-Hassan Moosavy, and Sahar Nouri Gharajalar, and Seyed Amin Khatibi
High prevalence of antibiotic resistance in pathogenic foodborne bacteria isolated from bovine milk.
March 2022, Scientific reports,
Maryam Rajaei, and Mir-Hassan Moosavy, and Sahar Nouri Gharajalar, and Seyed Amin Khatibi
Phenotypic and Genotypic Assessment of Antibiotic Resistance of Staphylococcus aureus Bacteria Isolated from Retail Meat.
January 2020, Infection and drug resistance,
Maryam Rajaei, and Mir-Hassan Moosavy, and Sahar Nouri Gharajalar, and Seyed Amin Khatibi
Occurrence of antibiotic resistance among Enterobacterales isolated from raw and ready-to-eat food - phenotypic and genotypic characteristics.
August 2022, International journal of environmental health research,
Maryam Rajaei, and Mir-Hassan Moosavy, and Sahar Nouri Gharajalar, and Seyed Amin Khatibi
Genotypic and phenotypic profiles of antibiotic-resistant bacteria isolated from hospitalised patients in Bangladesh.
July 2021, Tropical medicine & international health : TM & IH,
Maryam Rajaei, and Mir-Hassan Moosavy, and Sahar Nouri Gharajalar, and Seyed Amin Khatibi
Phenotypic and Genotypic Assessment of Antibiotic Resistance and Genotyping of vacA, cagA, iceA, oipA, cagE, and babA2 Alleles of Helicobacter pylori Bacteria Isolated from Raw Meat.
January 2020, Infection and drug resistance,
Maryam Rajaei, and Mir-Hassan Moosavy, and Sahar Nouri Gharajalar, and Seyed Amin Khatibi
Antibiotic resistance mechanism and diagnosis of common foodborne pathogens based on genotypic and phenotypic biomarkers.
July 2023, Comprehensive reviews in food science and food safety,
Maryam Rajaei, and Mir-Hassan Moosavy, and Sahar Nouri Gharajalar, and Seyed Amin Khatibi
Phenotypic analysis of antibiotic resistance and genotypic study of the vacA, cagA, iceA, oipA and babA genotypes of the Helicobacter pylori strains isolated from raw milk.
January 2018, Antimicrobial resistance and infection control,
Maryam Rajaei, and Mir-Hassan Moosavy, and Sahar Nouri Gharajalar, and Seyed Amin Khatibi
Antibiotic resistance pattern among gram-negative bacteria isolated from raw milk in Multan.
July 2005, Pakistan journal of pharmaceutical sciences,
Maryam Rajaei, and Mir-Hassan Moosavy, and Sahar Nouri Gharajalar, and Seyed Amin Khatibi
Phenotypic and genotypic detection of antibiotic resistance of Pseudomonas aeruginosa isolated from urinary tract infections.
March 2018, African health sciences,
Maryam Rajaei, and Mir-Hassan Moosavy, and Sahar Nouri Gharajalar, and Seyed Amin Khatibi
Phenotypic and genotypic antimicrobial resistance traits of foodborne Staphylococcus aureus isolates from Shanghai.
April 2014, Journal of food science,
Copied contents to your clipboard!