Whole-genome sequencing reveals high genetic diversity of Streptococcus uberis isolated from cows with mastitis. 2021

Nathália Cristina Cirone Silva, and Yongqiang Yang, and Marjory Xavier Rodrigues, and Tiago Tomazi, and Rodrigo Carvalho Bicalho
Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, New York, USA. ncirone@unicamp.br.

BACKGROUND Bovine mastitis is an important cause of economic loss in dairy farms. Streptococcus uberis is among the most frequently isolated bacterial species isolated from cows with mastitis. The aim of this study was to perform an in-depth genetic assessment of S. uberis strains isolated from bovine clinical mastitis (CM) and to perform a phylogenetic analysis to represent the evolutionary relationship among S. uberis sequences. RESULTS A total of 159 isolates was genetically characterized using whole genome sequencing. According to the virulence determinants, all strains harbored the hasC, leuS, perR, purH, and purN virulence genes. Thirty-four resistance genes were identified in at least one strain. In terms of acquired genes, we observed that 152 (95.6 %) strains had a resistance gene to lincosamine (lnuD), 48 (30.2 %) to tetracycline (tetM), 4 (2.51 %) to tobramicine (ant6), and 1 to lincosamide (lsa(E)). MLST detected the Sequence Type (ST)797 (n = 23), while 85.5 % of the strains did not match to known STs. CONCLUSIONS Then, eleven distinct ST were identified after we submitted the new alleles to assign new STs. The other prevalent STs observed were ST1215 (n = 58), ST1219 (n = 35), and ST1213 (n = 15). And it was not possible to identify the MLST of four strains. Phylogenetic lineages indicated a high genomic diversity of S. uberis in our collection, confirming that most strains isolated from bovine mastitis have different reservoirs, typical of environmental pathogens.

UI MeSH Term Description Entries
D008414 Mastitis, Bovine INFLAMMATION of the UDDER in cows. Bovine Mastitides,Bovine Mastitis,Mastitides, Bovine
D010802 Phylogeny The relationships of groups of organisms as reflected by their genetic makeup. Community Phylogenetics,Molecular Phylogenetics,Phylogenetic Analyses,Phylogenetic Analysis,Phylogenetic Clustering,Phylogenetic Comparative Analysis,Phylogenetic Comparative Methods,Phylogenetic Distance,Phylogenetic Generalized Least Squares,Phylogenetic Groups,Phylogenetic Incongruence,Phylogenetic Inference,Phylogenetic Networks,Phylogenetic Reconstruction,Phylogenetic Relatedness,Phylogenetic Relationships,Phylogenetic Signal,Phylogenetic Structure,Phylogenetic Tree,Phylogenetic Trees,Phylogenomics,Analyse, Phylogenetic,Analysis, Phylogenetic,Analysis, Phylogenetic Comparative,Clustering, Phylogenetic,Community Phylogenetic,Comparative Analysis, Phylogenetic,Comparative Method, Phylogenetic,Distance, Phylogenetic,Group, Phylogenetic,Incongruence, Phylogenetic,Inference, Phylogenetic,Method, Phylogenetic Comparative,Molecular Phylogenetic,Network, Phylogenetic,Phylogenetic Analyse,Phylogenetic Clusterings,Phylogenetic Comparative Analyses,Phylogenetic Comparative Method,Phylogenetic Distances,Phylogenetic Group,Phylogenetic Incongruences,Phylogenetic Inferences,Phylogenetic Network,Phylogenetic Reconstructions,Phylogenetic Relatednesses,Phylogenetic Relationship,Phylogenetic Signals,Phylogenetic Structures,Phylogenetic, Community,Phylogenetic, Molecular,Phylogenies,Phylogenomic,Reconstruction, Phylogenetic,Relatedness, Phylogenetic,Relationship, Phylogenetic,Signal, Phylogenetic,Structure, Phylogenetic,Tree, Phylogenetic
D002417 Cattle Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor. Beef Cow,Bos grunniens,Bos indicus,Bos indicus Cattle,Bos taurus,Cow,Cow, Domestic,Dairy Cow,Holstein Cow,Indicine Cattle,Taurine Cattle,Taurus Cattle,Yak,Zebu,Beef Cows,Bos indicus Cattles,Cattle, Bos indicus,Cattle, Indicine,Cattle, Taurine,Cattle, Taurus,Cattles, Bos indicus,Cattles, Indicine,Cattles, Taurine,Cattles, Taurus,Cow, Beef,Cow, Dairy,Cow, Holstein,Cows,Dairy Cows,Domestic Cow,Domestic Cows,Indicine Cattles,Taurine Cattles,Taurus Cattles,Yaks,Zebus
D005260 Female Females
D000073336 Whole Genome Sequencing Techniques to determine the entire sequence of the GENOME of an organism or individual. Complete Genome Sequencing,Genome Sequencing, Complete,Genome Sequencing, Whole,Sequencing, Complete Genome,Sequencing, Whole Genome
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D013290 Streptococcal Infections Infections with bacteria of the genus STREPTOCOCCUS. Group A Strep Infection,Group A Streptococcal Infection,Group A Streptococcal Infections,Group B Strep Infection,Group B Streptococcal Infection,Group B Streptococcal Infections,Infections, Streptococcal,Infection, Streptococcal,Streptococcal Infection
D013291 Streptococcus A genus of gram-positive, coccoid bacteria whose organisms occur in pairs or chains. No endospores are produced. Many species exist as commensals or parasites on man or animals with some being highly pathogenic. A few species are saprophytes and occur in the natural environment.
D014644 Genetic Variation Genotypic differences observed among individuals in a population. Genetic Diversity,Variation, Genetic,Diversity, Genetic,Diversities, Genetic,Genetic Diversities,Genetic Variations,Variations, Genetic
D014774 Virulence The degree of pathogenicity within a group or species of microorganisms or viruses as indicated by case fatality rates and/or the ability of the organism to invade the tissues of the host. The pathogenic capacity of an organism is determined by its VIRULENCE FACTORS. Pathogenicity

Related Publications

Nathália Cristina Cirone Silva, and Yongqiang Yang, and Marjory Xavier Rodrigues, and Tiago Tomazi, and Rodrigo Carvalho Bicalho
October 1998, Zentralblatt fur Veterinarmedizin. Reihe B. Journal of veterinary medicine. Series B,
Nathália Cristina Cirone Silva, and Yongqiang Yang, and Marjory Xavier Rodrigues, and Tiago Tomazi, and Rodrigo Carvalho Bicalho
January 2015, Revista Argentina de microbiologia,
Nathália Cristina Cirone Silva, and Yongqiang Yang, and Marjory Xavier Rodrigues, and Tiago Tomazi, and Rodrigo Carvalho Bicalho
January 2013, The Journal of general and applied microbiology,
Nathália Cristina Cirone Silva, and Yongqiang Yang, and Marjory Xavier Rodrigues, and Tiago Tomazi, and Rodrigo Carvalho Bicalho
April 2001, Journal of clinical microbiology,
Nathália Cristina Cirone Silva, and Yongqiang Yang, and Marjory Xavier Rodrigues, and Tiago Tomazi, and Rodrigo Carvalho Bicalho
January 2023, Frontiers in cellular and infection microbiology,
Nathália Cristina Cirone Silva, and Yongqiang Yang, and Marjory Xavier Rodrigues, and Tiago Tomazi, and Rodrigo Carvalho Bicalho
November 2023, Pathogens (Basel, Switzerland),
Nathália Cristina Cirone Silva, and Yongqiang Yang, and Marjory Xavier Rodrigues, and Tiago Tomazi, and Rodrigo Carvalho Bicalho
January 2017, Genome announcements,
Nathália Cristina Cirone Silva, and Yongqiang Yang, and Marjory Xavier Rodrigues, and Tiago Tomazi, and Rodrigo Carvalho Bicalho
March 2014, New Zealand veterinary journal,
Nathália Cristina Cirone Silva, and Yongqiang Yang, and Marjory Xavier Rodrigues, and Tiago Tomazi, and Rodrigo Carvalho Bicalho
January 2023, Frontiers in veterinary science,
Nathália Cristina Cirone Silva, and Yongqiang Yang, and Marjory Xavier Rodrigues, and Tiago Tomazi, and Rodrigo Carvalho Bicalho
September 2002, Journal of dairy science,
Copied contents to your clipboard!