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Sixteen analogues of ATP have been tested in the aminoacylation reaction of threonyl-tRNA, lysyl-tRNA, and arginyl-tRNA synthetases from baker's yeast. Two compounds are substrates for threonyl-tRNA and for lysyl-tRNA synthetases and five compounds for arginyl-tRNA synthetase. There are six inhibitors for threonyl-tRNA, nine for lysyl-tRNA, and six for arginyl-tRNA synthetase. Their Km and Ki values have been determined. Thus positions 2, 6, 7, 8 and 9 of the purine moiety and 2' and 3' of the sugar moiety of the ATP molecule are important for catalytic action of these aminoacyl-tRNA synthetases. Remarkably arginyl-tRNA synthetase is the first aminoacyl-tRNA synthetase which tolerates bulky substituents at the sugar moiety of ATP. These data fit with the idea that synthetases of subunit structure need magnesium-ion-ATP complexes with an anti conformation as substrates whereas single-chain enzymes accept this substrate in the syn conformation.
W Freist, and
H Sternbach, and
F von der Haar, and
F Cramer
January 1980,
European journal of biochemistry,
W Freist, and
H Sternbach, and
F von der Haar, and
F Cramer
May 1976,
European journal of biochemistry,
W Freist, and
H Sternbach, and
F von der Haar, and
F Cramer
July 1976,
European journal of biochemistry,
W Freist, and
H Sternbach, and
F von der Haar, and
F Cramer
October 1982,
Hoppe-Seyler's Zeitschrift fur physiologische Chemie,
W Freist, and
H Sternbach, and
F von der Haar, and
F Cramer
September 1982,
Biochemistry,
W Freist, and
H Sternbach, and
F von der Haar, and
F Cramer
April 1982,
Hoppe-Seyler's Zeitschrift fur physiologische Chemie,
W Freist, and
H Sternbach, and
F von der Haar, and
F Cramer
May 1976,
European journal of biochemistry,
W Freist, and
H Sternbach, and
F von der Haar, and
F Cramer
July 1975,
Biochemical and biophysical research communications,
W Freist, and
H Sternbach, and
F von der Haar, and
F Cramer
June 1979,
Biochemical and biophysical research communications,
W Freist, and
H Sternbach, and
F von der Haar, and
F Cramer
July 1978,
Biochemistry,
