BIOMAT Database Logo BIOMAT Database Logo

Ursolic Acid Targets Glucosyltransferase and Inhibits Its Activity to Prevent Streptococcus mutans Biofilm Formation. 2021

Yucui Liu, and Yanxin Huang, and Cong Fan, and Zhongmei Chi, and Miao Bai, and Luguo Sun, and Li Yang, and Chunlei Yu, and Zhenbo Song, and Xiaoguang Yang, and Jingwen Yi, and Shuyue Wang, and Lei Liu, and Guannan Wang, and Lihua Zheng
National Engineering Laboratory for Druggable Gene and Protein Screening, Northeast Normal University, Changchun, China.

Streptococcus mutans (S. mutans), the prime pathogen of dental caries, can secrete glucosyltransferases (GTFs) to synthesize extracellular polysaccharides (EPSs), which are the virulence determinants of cariogenic biofilms. Ursolic acid, a type of pentacyclic triterpene natural compound, has shown potential antibiofilm effects on S. mutans. To investigate the mechanisms of ursolic acid-mediated inhibition of S. mutans biofilm formation, we first demonstrated that ursolic acid could decrease the viability and structural integrity of biofilms, as evidenced by XTT, crystal violet, and live/dead staining assays. Then, we further revealed that ursolic acid could compete with the inherent substrate to occupy the catalytic center of GTFs to inhibit EPS formation, and this was confirmed by GTF activity assays, computer simulations, site-directed mutagenesis, and capillary electrophoresis (CE). In conclusion, ursolic acid can decrease bacterial viability and prevent S. mutans biofilm formation by binding and inhibiting the activity of GTFs.

UI MeSH Term Description Entries

Related Publications

Yucui Liu, and Yanxin Huang, and Cong Fan, and Zhongmei Chi, and Miao Bai, and Luguo Sun, and Li Yang, and Chunlei Yu, and Zhenbo Song, and Xiaoguang Yang, and Jingwen Yi, and Shuyue Wang, and Lei Liu, and Guannan Wang, and Lihua Zheng
Raffinose Inhibits Streptococcus mutans Biofilm Formation by Targeting Glucosyltransferase.
June 2022, Microbiology spectrum,
Yucui Liu, and Yanxin Huang, and Cong Fan, and Zhongmei Chi, and Miao Bai, and Luguo Sun, and Li Yang, and Chunlei Yu, and Zhenbo Song, and Xiaoguang Yang, and Jingwen Yi, and Shuyue Wang, and Lei Liu, and Guannan Wang, and Lihua Zheng
Molecule Targeting Glucosyltransferase Inhibits Streptococcus mutans Biofilm Formation and Virulence.
January 2016, Antimicrobial agents and chemotherapy,
Yucui Liu, and Yanxin Huang, and Cong Fan, and Zhongmei Chi, and Miao Bai, and Luguo Sun, and Li Yang, and Chunlei Yu, and Zhenbo Song, and Xiaoguang Yang, and Jingwen Yi, and Shuyue Wang, and Lei Liu, and Guannan Wang, and Lihua Zheng
Inhibition of glucosyltransferase from Streptococcus mutans by oleanolic acid and ursolic acid.
January 1987, Caries research,
Yucui Liu, and Yanxin Huang, and Cong Fan, and Zhongmei Chi, and Miao Bai, and Luguo Sun, and Li Yang, and Chunlei Yu, and Zhenbo Song, and Xiaoguang Yang, and Jingwen Yi, and Shuyue Wang, and Lei Liu, and Guannan Wang, and Lihua Zheng
Inhibition of Streptococcus mutans biofilm formation on composite resins containing ursolic acid.
May 2013, Restorative dentistry & endodontics,
Yucui Liu, and Yanxin Huang, and Cong Fan, and Zhongmei Chi, and Miao Bai, and Luguo Sun, and Li Yang, and Chunlei Yu, and Zhenbo Song, and Xiaoguang Yang, and Jingwen Yi, and Shuyue Wang, and Lei Liu, and Guannan Wang, and Lihua Zheng
Lactobacillus plantarum lipoteichoic acid inhibits biofilm formation of Streptococcus mutans.
January 2018, PloS one,
Yucui Liu, and Yanxin Huang, and Cong Fan, and Zhongmei Chi, and Miao Bai, and Luguo Sun, and Li Yang, and Chunlei Yu, and Zhenbo Song, and Xiaoguang Yang, and Jingwen Yi, and Shuyue Wang, and Lei Liu, and Guannan Wang, and Lihua Zheng
Kaurenoic Acid from Aralia continentalis Inhibits Biofilm Formation of Streptococcus mutans.
January 2013, Evidence-based complementary and alternative medicine : eCAM,
Yucui Liu, and Yanxin Huang, and Cong Fan, and Zhongmei Chi, and Miao Bai, and Luguo Sun, and Li Yang, and Chunlei Yu, and Zhenbo Song, and Xiaoguang Yang, and Jingwen Yi, and Shuyue Wang, and Lei Liu, and Guannan Wang, and Lihua Zheng
Ursolic acid inhibits multi-species biofilms developed by Streptococcus mutans, Streptococcus sanguinis, and Streptococcus gordonii.
May 2021, Archives of oral biology,
Yucui Liu, and Yanxin Huang, and Cong Fan, and Zhongmei Chi, and Miao Bai, and Luguo Sun, and Li Yang, and Chunlei Yu, and Zhenbo Song, and Xiaoguang Yang, and Jingwen Yi, and Shuyue Wang, and Lei Liu, and Guannan Wang, and Lihua Zheng
Thermophilin 110 inhibits growth and biofilm formation of Streptococcus mutans.
September 2021, Biotechnology reports (Amsterdam, Netherlands),
Yucui Liu, and Yanxin Huang, and Cong Fan, and Zhongmei Chi, and Miao Bai, and Luguo Sun, and Li Yang, and Chunlei Yu, and Zhenbo Song, and Xiaoguang Yang, and Jingwen Yi, and Shuyue Wang, and Lei Liu, and Guannan Wang, and Lihua Zheng
Quantitative acetylome analysis reveals involvement of glucosyltransferase acetylation in Streptococcus mutans biofilm formation.
April 2021, Environmental microbiology reports,
Yucui Liu, and Yanxin Huang, and Cong Fan, and Zhongmei Chi, and Miao Bai, and Luguo Sun, and Li Yang, and Chunlei Yu, and Zhenbo Song, and Xiaoguang Yang, and Jingwen Yi, and Shuyue Wang, and Lei Liu, and Guannan Wang, and Lihua Zheng
Essential oil of Curcuma longa inhibits Streptococcus mutans biofilm formation.
January 2011, Journal of food science,
Copied contents to your clipboard!