OBJECTIVE To evaluate the performance of precorneal and prelens non-invasive tear break-up time (NIBUT) measurements to determine tear instability in contact lens (CL) wearers. METHODS In this study, 50 eyes of 25 CL wearers were evaluated. Precorneal first and average NIBUTs and fluorescein tear break-up time (FBUT) were measured before wearing CLs in the morning. Those with FBUT less than 10 s were considered to have tear instability. After wearing CL, first and average prelens NIBUTs were measured at the 30th minute. The receiver operating characteristic (ROC) curve was analyzed to evaluate the performance of NIBUT measurements in the diagnosis of tear instability. RESULTS The FBUT value had a significant correlation with first and average precorneal NIBUT values (p < 0.001, r = 0.653 and p < 0.001, r = 0.628, respectively). The FBUT value had no correlation with the prelens first and average NIBUT values (p = 0.542 and p = 0.263, respectively). To understand the relationship between the precorneal and prelens NIBUT values measured by the automated method, their correlation was evaluated. There was no significant correlation between the precorneal and prelens NIBUTs (for all; p > 0.05). The area under curve (AUC) in ROC curve for the first and average precorneal NIBUTs were 0.842 (p < 0.001) and 0.810 (p < 0.001), respectively. The AUC values for the first and average prelens NIBUTs at the 30th minute of CL wear were 0.586 and 0.619, respectively (p = 0.317 and p = 0.166, respectively). CONCLUSIONS Precorneal NIBUT measurements may be useful in diagnosing tear instability. Prelens NIBUT values are not yet capable of adequately defining the tear film dynamics in CL wearers.