Protective Effects of Crocetin on Arsenic Trioxide-induced Oxidative Stress in Human Umbilical Vein Endothelial Cells. 2021

Chung-Lin Tsai, and Chia-Wen Tsai, and Wen-Shin Chang, and Jiunn-Cherng Lin, and Liang-Chun Shih, and Jie-Long He, and DA-Tian Bau
Graduate Institute of Clinical Medical Science, China Medical University, Taichung, Taiwan, R.O.C.

OBJECTIVE The clinical use of arsenic trioxide (As2O3) is hampered due to its cardiotoxicity. Therefore, it is critical to prevent As2O3-induced loss of endothelial integrity. The purpose of this study was to examine As2O3-induced endothelial dysfunction and evaluate the efficacy of crocetin on reversing As2O3-induced cardiotoxicity. METHODS Cultured human umbilical vein endothelial cells (HUVECs) were used to examine As2O3-induced oxidative stress, apoptosis, production of reactive oxygen species (ROS) and DNA adducts. In addition, the impact of crocetin on As2O3-induced cardiotoxicity was evaluated. RESULTS As2O3 decreased the viability of HUVEC cells and led to apoptosis. Additionally, As2O3 elevated NADPH oxidase activity, and the levels of intracellular ROS. Furthermore, the formamidopyrimidine DNA-glycosylase- and endonuclease III-digestible adducts were induced by As2O3 Crocetin treatment reversed the As2O3-induced reduction in cell viability, the induction of apoptosis, the activation of NADPH oxidase activity, ROS levels and DNA adducts. CONCLUSIONS Crocetin protects from As2O3-induced cardio-toxicity.

UI MeSH Term Description Entries
D010087 Oxides Binary compounds of oxygen containing the anion O(2-). The anion combines with metals to form alkaline oxides and non-metals to form acidic oxides. Oxide
D002338 Carotenoids The general name for a group of fat-soluble pigments found in green, yellow, and leafy vegetables, and yellow fruits. They are aliphatic hydrocarbons containing 4 terpene subunits. Carotenes,Carotenoid,Tetraterpene Derivatives,Tetraterpenes,Carotene,Derivatives, Tetraterpene
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000077237 Arsenic Trioxide An inorganic compound with the chemical formula As2O3 that is used for the treatment of ACUTE PROMYELOCYTIC LEUKEMIA in patients who have relapsed from, or are resistant to, conventional drug therapy. Arsenic Oxide (As2O3),Arsenic Oxide (As4O6),Arsenic(III) Oxide,Arsenolite,Arsenous Anhydride,As2O3,As4O6,Diarsenic Trioxide,Naonobin,Tetra-Arsenic Hexaoxide,Tetra-Arsenic Oxide,Tetraarsenic Hexaoxide,Tetraarsenic Oxide,Trisenox,Trixenox,Tetra Arsenic Hexaoxide,Tetra Arsenic Oxide
D001152 Arsenicals Inorganic or organic compounds that contain arsenic. Arsenic Compounds,Compounds, Arsenic
D014801 Vitamin A Retinol and derivatives of retinol that play an essential role in metabolic functioning of the retina, the growth of and differentiation of epithelial tissue, the growth of bone, reproduction, and the immune response. Dietary vitamin A is derived from a variety of CAROTENOIDS found in plants. It is enriched in the liver, egg yolks, and the fat component of dairy products. Retinol,11-cis-Retinol,3,7-dimethyl-9-(2,6,6-trimethyl-1-cyclohexen-1-yl)-2,4,6,8-nonatetraen-1-ol, (all-E)-Isomer,All-Trans-Retinol,Aquasol A,Vitamin A1,All Trans Retinol
D017209 Apoptosis A regulated cell death mechanism characterized by distinctive morphologic changes in the nucleus and cytoplasm, including the endonucleolytic cleavage of genomic DNA, at regularly spaced, internucleosomal sites, i.e., DNA FRAGMENTATION. It is genetically programmed and serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth. Apoptosis, Extrinsic Pathway,Apoptosis, Intrinsic Pathway,Caspase-Dependent Apoptosis,Classic Apoptosis,Classical Apoptosis,Programmed Cell Death,Programmed Cell Death, Type I,Apoptoses, Extrinsic Pathway,Apoptoses, Intrinsic Pathway,Apoptosis, Caspase-Dependent,Apoptosis, Classic,Apoptosis, Classical,Caspase Dependent Apoptosis,Cell Death, Programmed,Classic Apoptoses,Extrinsic Pathway Apoptoses,Extrinsic Pathway Apoptosis,Intrinsic Pathway Apoptoses,Intrinsic Pathway Apoptosis
D017382 Reactive Oxygen Species Molecules or ions formed by the incomplete one-electron reduction of oxygen. These reactive oxygen intermediates include SINGLET OXYGEN; SUPEROXIDES; PEROXIDES; HYDROXYL RADICAL; and HYPOCHLOROUS ACID. They contribute to the microbicidal activity of PHAGOCYTES, regulation of SIGNAL TRANSDUCTION and GENE EXPRESSION, and the oxidative damage to NUCLEIC ACIDS; PROTEINS; and LIPIDS. Active Oxygen Species,Oxygen Radical,Oxygen Radicals,Pro-Oxidant,Reactive Oxygen Intermediates,Active Oxygen,Oxygen Species, Reactive,Pro-Oxidants,Oxygen, Active,Pro Oxidant,Pro Oxidants,Radical, Oxygen
D061307 Human Umbilical Vein Endothelial Cells Endothelial cells that line venous vessels of the UMBILICAL CORD. Human Umbilical Vein Endothelial Cell,Endothelial Cells, Human Umbilical Vein,HUVEC Cells,Cell, HUVEC,Cells, HUVEC,HUVEC Cell
D018384 Oxidative Stress A disturbance in the prooxidant-antioxidant balance in favor of the former, leading to potential damage. Indicators of oxidative stress include damaged DNA bases, protein oxidation products, and lipid peroxidation products (Sies, Oxidative Stress, 1991, pxv-xvi). Anti-oxidative Stress,Antioxidative Stress,DNA Oxidative Damage,Nitro-Oxidative Stress,Oxidative Cleavage,Oxidative DNA Damage,Oxidative Damage,Oxidative Injury,Oxidative Nitrative Stress,Oxidative Stress Injury,Oxidative and Nitrosative Stress,Stress, Oxidative,Anti oxidative Stress,Anti-oxidative Stresses,Antioxidative Stresses,Cleavage, Oxidative,DNA Damage, Oxidative,DNA Oxidative Damages,Damage, DNA Oxidative,Damage, Oxidative,Damage, Oxidative DNA,Injury, Oxidative,Injury, Oxidative Stress,Nitrative Stress, Oxidative,Nitro Oxidative Stress,Nitro-Oxidative Stresses,Oxidative Cleavages,Oxidative DNA Damages,Oxidative Damage, DNA,Oxidative Damages,Oxidative Injuries,Oxidative Nitrative Stresses,Oxidative Stress Injuries,Oxidative Stresses,Stress Injury, Oxidative,Stress, Anti-oxidative,Stress, Antioxidative,Stress, Nitro-Oxidative,Stress, Oxidative Nitrative,Stresses, Nitro-Oxidative

Related Publications

Chung-Lin Tsai, and Chia-Wen Tsai, and Wen-Shin Chang, and Jiunn-Cherng Lin, and Liang-Chun Shih, and Jie-Long He, and DA-Tian Bau
August 2018, Experimental and therapeutic medicine,
Chung-Lin Tsai, and Chia-Wen Tsai, and Wen-Shin Chang, and Jiunn-Cherng Lin, and Liang-Chun Shih, and Jie-Long He, and DA-Tian Bau
June 2011, Canadian journal of physiology and pharmacology,
Chung-Lin Tsai, and Chia-Wen Tsai, and Wen-Shin Chang, and Jiunn-Cherng Lin, and Liang-Chun Shih, and Jie-Long He, and DA-Tian Bau
February 2013, European journal of pharmacology,
Chung-Lin Tsai, and Chia-Wen Tsai, and Wen-Shin Chang, and Jiunn-Cherng Lin, and Liang-Chun Shih, and Jie-Long He, and DA-Tian Bau
January 2016, Zeitschrift fur Naturforschung. C, Journal of biosciences,
Chung-Lin Tsai, and Chia-Wen Tsai, and Wen-Shin Chang, and Jiunn-Cherng Lin, and Liang-Chun Shih, and Jie-Long He, and DA-Tian Bau
January 2016, Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology,
Chung-Lin Tsai, and Chia-Wen Tsai, and Wen-Shin Chang, and Jiunn-Cherng Lin, and Liang-Chun Shih, and Jie-Long He, and DA-Tian Bau
March 2019, Journal of food biochemistry,
Chung-Lin Tsai, and Chia-Wen Tsai, and Wen-Shin Chang, and Jiunn-Cherng Lin, and Liang-Chun Shih, and Jie-Long He, and DA-Tian Bau
December 2011, Food chemistry,
Chung-Lin Tsai, and Chia-Wen Tsai, and Wen-Shin Chang, and Jiunn-Cherng Lin, and Liang-Chun Shih, and Jie-Long He, and DA-Tian Bau
March 2010, Toxicology in vitro : an international journal published in association with BIBRA,
Chung-Lin Tsai, and Chia-Wen Tsai, and Wen-Shin Chang, and Jiunn-Cherng Lin, and Liang-Chun Shih, and Jie-Long He, and DA-Tian Bau
January 2010, Journal of the science of food and agriculture,
Chung-Lin Tsai, and Chia-Wen Tsai, and Wen-Shin Chang, and Jiunn-Cherng Lin, and Liang-Chun Shih, and Jie-Long He, and DA-Tian Bau
January 2008, Mediators of inflammation,
Copied contents to your clipboard!