BIOMAT Database Logo BIOMAT Database Logo

Searching for gene defects by denaturing gradient gel electrophoresis. 1986

L S Lerman, and K Silverstein, and E Grinfeld

UI MeSH Term Description Entries
D008957 Models, Genetic Theoretical representations that simulate the behavior or activity of genetic processes or phenomena. They include the use of mathematical equations, computers, and other electronic equipment. Genetic Models,Genetic Model,Model, Genetic
D009154 Mutation Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations. Mutations
D009691 Nucleic Acid Denaturation Disruption of the secondary structure of nucleic acids by heat, extreme pH or chemical treatment. Double strand DNA is "melted" by dissociation of the non-covalent hydrogen bonds and hydrophobic interactions. Denatured DNA appears to be a single-stranded flexible structure. The effects of denaturation on RNA are similar though less pronounced and largely reversible. DNA Denaturation,DNA Melting,RNA Denaturation,Acid Denaturation, Nucleic,Denaturation, DNA,Denaturation, Nucleic Acid,Denaturation, RNA,Nucleic Acid Denaturations
D009693 Nucleic Acid Hybridization Widely used technique which exploits the ability of complementary sequences in single-stranded DNAs or RNAs to pair with each other to form a double helix. Hybridization can take place between two complimentary DNA sequences, between a single-stranded DNA and a complementary RNA, or between two RNA sequences. The technique is used to detect and isolate specific sequences, measure homology, or define other characteristics of one or both strands. (Kendrew, Encyclopedia of Molecular Biology, 1994, p503) Genomic Hybridization,Acid Hybridization, Nucleic,Acid Hybridizations, Nucleic,Genomic Hybridizations,Hybridization, Genomic,Hybridization, Nucleic Acid,Hybridizations, Genomic,Hybridizations, Nucleic Acid,Nucleic Acid Hybridizations
D004247 DNA A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine). DNA, Double-Stranded,Deoxyribonucleic Acid,ds-DNA,DNA, Double Stranded,Double-Stranded DNA,ds DNA
D004586 Electrophoresis An electrochemical process in which macromolecules or colloidal particles with a net electric charge migrate in a solution under the influence of an electric current. Electrophoreses
D005796 Genes A category of nucleic acid sequences that function as units of heredity and which code for the basic instructions for the development, reproduction, and maintenance of organisms. Cistron,Gene,Genetic Materials,Cistrons,Genetic Material,Material, Genetic,Materials, Genetic
D005914 Globins A superfamily of proteins containing the globin fold which is composed of 6-8 alpha helices arranged in a characterstic HEME enclosing structure. Globin
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man

Related Publications

L S Lerman, and K Silverstein, and E Grinfeld
Denaturing gradient gel electrophoresis.
January 2002, Methods in molecular biology (Clifton, N.J.),
L S Lerman, and K Silverstein, and E Grinfeld
Detecting prion protein gene mutations by denaturing gradient gel electrophoresis.
January 1994, Human mutation,
L S Lerman, and K Silverstein, and E Grinfeld
Denaturing gradient gel electrophoresis (DGGE).
January 2013, Methods in molecular biology (Clifton, N.J.),
L S Lerman, and K Silverstein, and E Grinfeld
Denaturing gradient gel electrophoresis (DGGE).
February 2007, Journal of visualized experiments : JoVE,
L S Lerman, and K Silverstein, and E Grinfeld
Polymorphism in the APNH gene, detected by denaturing gradient gel electrophoresis.
September 1990, Nucleic acids research,
L S Lerman, and K Silverstein, and E Grinfeld
Detection of mutations by denaturing gradient gel electrophoresis.
May 2001, Current protocols in human genetics,
L S Lerman, and K Silverstein, and E Grinfeld
Mutation detection by denaturing gradient gel electrophoresis (DGGE).
January 1994, Human mutation,
L S Lerman, and K Silverstein, and E Grinfeld
[Electrophoresis in denaturing gradient gel (DGGE)].
March 1998, Bulletin du cancer,
L S Lerman, and K Silverstein, and E Grinfeld
Screening of alpha-1-antitrypsin gene by denaturing gradient gel electrophoresis (DGGE).
October 2006, Journal of biochemical and biophysical methods,
L S Lerman, and K Silverstein, and E Grinfeld
Detection of mutations in insulin receptor gene by denaturing gradient gel electrophoresis.
April 1992, Diabetes,
Copied contents to your clipboard!