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Purified preparations of the tRNA methylase deficient in supK strains of Salmonella typhimurium transfer methyl groups from S-adenosylmethionine (SAM) to at least two tRNA species, an alanine tRNA and a serine tRNA. The identity of the tRNA substrates for this enzyme was determined by a change in the elution position of the methyl-labeled tRNA from BND-cellulose columns before and after aminoacylation with a specific amino acid followed by derivatization of the free primary amino group with phenoxy- or naphthoxyacetate. The radioactive methyl group enzymatically added to these tRNAs is both acid and base labile and can be hydrolyzed to a volatile product at pHs above 7.5 and also at pH 1. The methylated 3'-nucleotide isolated from digested tRNA is a pyrimidine derivative and chromatographs like a modified uridylic acid. Its identity has not been established, but it is likely that it corresponds to the methyl ester of V, uridin-5-oxyacetic acid.
W T Pope, and
A Brown, and
R H Reeves
August 1976,
Nucleic acids research,
W T Pope, and
A Brown, and
R H Reeves
March 1970,
Life sciences. Pt. 2: Biochemistry, general and molecular biology,
W T Pope, and
A Brown, and
R H Reeves
February 1971,
Biochemical and biophysical research communications,
W T Pope, and
A Brown, and
R H Reeves
January 2015,
Methods in enzymology,
W T Pope, and
A Brown, and
R H Reeves
January 1979,
Mikrobiologiia,
W T Pope, and
A Brown, and
R H Reeves
May 1987,
Journal of biochemistry,
W T Pope, and
A Brown, and
R H Reeves
October 1972,
Biochimica et biophysica acta,
W T Pope, and
A Brown, and
R H Reeves
January 1978,
Physiological chemistry and physics,
W T Pope, and
A Brown, and
R H Reeves
April 1973,
Virology,
