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The methods for establishing osteoblast-rich rat calvarial cell cultures have been described, together with methods for the use of clonal osteogenic sarcoma cells of osteoblast phenotype. The latter clonal lines are useful for several purposes, but all the precautions and quality control measures necessary in the study of clonal lines must be observed. Some of the techniques for studying biochemical responses to hormones in these cells have also been detailed, but clearly others are applicable, including studies of the synthesis of matrix constituents. Osteoclast-like cells have not been considered in this chapter, because osteoclast culture methods have not yet been developed to the degree of purity and reproducibility necessary for this type of biochemical approach.
T J Martin, and
K W Ng, and
N C Partridge, and
S A Livesey
January 2005,
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T J Martin, and
K W Ng, and
N C Partridge, and
S A Livesey
November 1987,
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T J Martin, and
K W Ng, and
N C Partridge, and
S A Livesey
January 1983,
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T J Martin, and
K W Ng, and
N C Partridge, and
S A Livesey
January 1977,
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T J Martin, and
K W Ng, and
N C Partridge, and
S A Livesey
January 1987,
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T J Martin, and
K W Ng, and
N C Partridge, and
S A Livesey
May 1978,
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T J Martin, and
K W Ng, and
N C Partridge, and
S A Livesey
August 1963,
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T J Martin, and
K W Ng, and
N C Partridge, and
S A Livesey
February 1971,
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T J Martin, and
K W Ng, and
N C Partridge, and
S A Livesey
April 1966,
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T J Martin, and
K W Ng, and
N C Partridge, and
S A Livesey
July 2018,
The Malaysian journal of medical sciences : MJMS,
