-transparent.png){kind=logo}
Leukemic blast growth factors (LBGFs) are necessary for in vitro growth of clonogenic cells from patients with acute myeloblastic leukemia. As the human bladder carcinoma cell line 5637 had previously been reported to secrete abundant LBGFs into the culture supernatant, the LBGFs in 5637-conditioned medium (5637-CM) were characterized. Measurement of LBGFs was done using an in vitro leukemic blast colony assay in methylcellulose culture. LBGFs in 5637-CM were fractionated by anion exchange chromatography, and two peaks of activity were recovered. Pool B (high-salt eluent) and/or purified granulocyte colony-stimulating factor (G-CSF) were added to the clonogenic leukemic blast cell assays. It was found that pool B was more active than G-CSF in the majority of cases examined and that the two types of activity were synergistic in some cases.
Y Asano, and
T Shibuya, and
S Okamura, and
E Morioka, and
S Taniguchi, and
Y Niho
September 1985,
Blood,
Y Asano, and
T Shibuya, and
S Okamura, and
E Morioka, and
S Taniguchi, and
Y Niho
January 1990,
Haematologia,
Y Asano, and
T Shibuya, and
S Okamura, and
E Morioka, and
S Taniguchi, and
Y Niho
January 1990,
Research in experimental medicine. Zeitschrift fur die gesamte experimentelle Medizin einschliesslich experimenteller Chirurgie,
Y Asano, and
T Shibuya, and
S Okamura, and
E Morioka, and
S Taniguchi, and
Y Niho
April 1997,
Leukemia research,
Y Asano, and
T Shibuya, and
S Okamura, and
E Morioka, and
S Taniguchi, and
Y Niho
October 1995,
Leukemia,
Y Asano, and
T Shibuya, and
S Okamura, and
E Morioka, and
S Taniguchi, and
Y Niho
January 2011,
Urologic oncology,
Y Asano, and
T Shibuya, and
S Okamura, and
E Morioka, and
S Taniguchi, and
Y Niho
August 2008,
FEMS microbiology letters,
Y Asano, and
T Shibuya, and
S Okamura, and
E Morioka, and
S Taniguchi, and
Y Niho
April 1986,
Proceedings of the National Academy of Sciences of the United States of America,
Y Asano, and
T Shibuya, and
S Okamura, and
E Morioka, and
S Taniguchi, and
Y Niho
January 1985,
International journal of cell cloning,
Y Asano, and
T Shibuya, and
S Okamura, and
E Morioka, and
S Taniguchi, and
Y Niho
October 1987,
[Rinsho ketsueki] The Japanese journal of clinical hematology,
