Limited replication of human cytomegalovirus in a trophoblast cell line. 2021

Kadeem Hyde, and Nowshin Sultana, and Andy C Tran, and Narina Bileckaja, and Claire L Donald, and Alain Kohl, and Richard J Stanton, and Blair L Strang
Institute for Infection and Immunity, St George's, University of London, London, UK.

Several viruses, including human cytomegalovirus (HCMV), are thought to replicate in the placenta. However, there is little understanding of the molecular mechanisms involved in HCMV replication in this tissue. We investigated replication of HCMV in the extravillous trophoblast cell line SGHPL-4, a commonly used model of HCMV replication in the placenta. We found limited HCMV protein expression and virus replication in SGHPL-4 cells. This was associated with a lack of trophoblast progenitor cell protein markers in SGHPL-4 cells, suggesting a relationship between trophoblast differentiation and limited HCMV replication. We proposed that limited HCMV replication in trophoblast cells is advantageous to vertical transmission of HCMV, as there is a greater opportunity for vertical transmission when the placenta is intact and functional. Furthermore, when we investigated the replication of other vertically transmitted viruses in SGHPL-4 cells we found some limitation to replication of Zika virus, but not herpes simplex virus. Thus, limited replication of some, but not all, vertically transmitted viruses may be a feature of trophoblast cells.

UI MeSH Term Description Entries
D010920 Placenta A highly vascularized mammalian fetal-maternal organ and major site of transport of oxygen, nutrients, and fetal waste products. It includes a fetal portion (CHORIONIC VILLI) derived from TROPHOBLASTS and a maternal portion (DECIDUA) derived from the uterine ENDOMETRIUM. The placenta produces an array of steroid, protein and peptide hormones (PLACENTAL HORMONES). Placentoma, Normal,Placentome,Placentas,Placentomes
D011247 Pregnancy The status during which female mammals carry their developing young (EMBRYOS or FETUSES) in utero before birth, beginning from FERTILIZATION to BIRTH. Gestation,Pregnancies
D002460 Cell Line Established cell cultures that have the potential to propagate indefinitely. Cell Lines,Line, Cell,Lines, Cell
D003586 Cytomegalovirus Infections Infection with CYTOMEGALOVIRUS, characterized by enlarged cells bearing intranuclear inclusions. Infection may be in almost any organ, but the salivary glands are the most common site in children, as are the lungs in adults. CMV Inclusion,CMV Inclusions,Congenital CMV Infection,Congenital Cytomegalovirus Infection,Cytomegalic Inclusion Disease,Cytomegalovirus Colitis,Cytomegalovirus Inclusion,Cytomegalovirus Inclusion Disease,Cytomegalovirus Inclusions,Inclusion Disease,Perinatal CMV Infection,Perinatal Cytomegalovirus Infection,Renal Tubular Cytomegalovirus Inclusion,Renal Tubular Cytomegalovirus Inclusions,Salivary Gland Virus Disease,Severe Cytomegalovirus Infection,Severe Cytomegalovirus Infections,Infections, Cytomegalovirus,CMV Infection, Congenital,CMV Infection, Perinatal,Colitis, Cytomegalovirus,Congenital CMV Infections,Congenital Cytomegalovirus Infections,Cytomegalic Inclusion Diseases,Cytomegalovirus Colitides,Cytomegalovirus Inclusion Diseases,Cytomegalovirus Infection,Cytomegalovirus Infection, Congenital,Cytomegalovirus Infection, Perinatal,Cytomegalovirus Infection, Severe,Cytomegalovirus Infections, Severe,Disease, Cytomegalic Inclusion,Disease, Cytomegalovirus Inclusion,Diseases, Cytomegalovirus Inclusion,Inclusion Disease, Cytomegalic,Inclusion Disease, Cytomegalovirus,Inclusion Diseases,Inclusion Diseases, Cytomegalovirus,Inclusion, CMV,Inclusion, Cytomegalovirus,Infection, Congenital CMV,Infection, Congenital Cytomegalovirus,Infection, Cytomegalovirus,Infection, Perinatal CMV,Infection, Perinatal Cytomegalovirus,Infection, Severe Cytomegalovirus,Perinatal CMV Infections,Perinatal Cytomegalovirus Infections
D003587 Cytomegalovirus A genus of the family HERPESVIRIDAE, subfamily BETAHERPESVIRINAE, infecting the salivary glands, liver, spleen, lungs, eyes, and other organs, in which they produce characteristically enlarged cells with intranuclear inclusions. Infection with Cytomegalovirus is also seen as an opportunistic infection in AIDS. Herpesvirus 5, Human,Human Herpesvirus 5,Salivary Gland Viruses,HHV 5,Herpesvirus 5 (beta), Human,Cytomegaloviruses,Salivary Gland Virus,Virus, Salivary Gland,Viruses, Salivary Gland
D005260 Female Females
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D014327 Trophoblasts Cells lining the outside of the BLASTOCYST. After binding to the ENDOMETRIUM, trophoblasts develop into two distinct layers, an inner layer of mononuclear cytotrophoblasts and an outer layer of continuous multinuclear cytoplasm, the syncytiotrophoblasts, which form the early fetal-maternal interface (PLACENTA). Cytotrophoblasts,Syncytiotrophoblasts,Trophoblast,Cytotrophoblast,Syncytiotrophoblast
D014779 Virus Replication The process of intracellular viral multiplication, consisting of the synthesis of PROTEINS; NUCLEIC ACIDS; and sometimes LIPIDS, and their assembly into a new infectious particle. Viral Replication,Replication, Viral,Replication, Virus,Replications, Viral,Replications, Virus,Viral Replications,Virus Replications
D018445 Infectious Disease Transmission, Vertical The transmission of infectious disease or pathogens from one generation to another. It includes transmission in utero or intrapartum by exposure to blood and secretions, and postpartum exposure via breastfeeding. Fetomaternal Infection Transmission,Infection Transmission, Fetomaternal,Infection Transmission, Maternal-Fetal,Infection Transmission, Vertical,Maternal-Fetal Infection Transmission,Mother-to-Child Transmission,Pathogen Transmission, Vertical,Vertical Infection Transmission,Vertical Infectious Disease Transmission,Vertical Transmission of Infectious Disease,Infection Transmission, Maternal Fetal,Maternal Fetal Infection Transmission,Mother to Child Transmission,Mother-to-Child Transmissions,Transmission, Fetomaternal Infection,Transmission, Maternal-Fetal Infection,Transmission, Mother-to-Child,Transmission, Vertical Infection,Transmission, Vertical Pathogen,Transmissions, Mother-to-Child,Vertical Pathogen Transmission

Related Publications

Kadeem Hyde, and Nowshin Sultana, and Andy C Tran, and Narina Bileckaja, and Claire L Donald, and Alain Kohl, and Richard J Stanton, and Blair L Strang
April 2024, Journal of virology,
Kadeem Hyde, and Nowshin Sultana, and Andy C Tran, and Narina Bileckaja, and Claire L Donald, and Alain Kohl, and Richard J Stanton, and Blair L Strang
December 2023, bioRxiv : the preprint server for biology,
Kadeem Hyde, and Nowshin Sultana, and Andy C Tran, and Narina Bileckaja, and Claire L Donald, and Alain Kohl, and Richard J Stanton, and Blair L Strang
January 1976, Archives of virology,
Kadeem Hyde, and Nowshin Sultana, and Andy C Tran, and Narina Bileckaja, and Claire L Donald, and Alain Kohl, and Richard J Stanton, and Blair L Strang
November 1991, Virology,
Kadeem Hyde, and Nowshin Sultana, and Andy C Tran, and Narina Bileckaja, and Claire L Donald, and Alain Kohl, and Richard J Stanton, and Blair L Strang
November 1994, Journal of virology,
Kadeem Hyde, and Nowshin Sultana, and Andy C Tran, and Narina Bileckaja, and Claire L Donald, and Alain Kohl, and Richard J Stanton, and Blair L Strang
November 1991, In vitro cellular & developmental biology : journal of the Tissue Culture Association,
Kadeem Hyde, and Nowshin Sultana, and Andy C Tran, and Narina Bileckaja, and Claire L Donald, and Alain Kohl, and Richard J Stanton, and Blair L Strang
January 1992, Medical microbiology and immunology,
Kadeem Hyde, and Nowshin Sultana, and Andy C Tran, and Narina Bileckaja, and Claire L Donald, and Alain Kohl, and Richard J Stanton, and Blair L Strang
August 2002, Biochemical and biophysical research communications,
Kadeem Hyde, and Nowshin Sultana, and Andy C Tran, and Narina Bileckaja, and Claire L Donald, and Alain Kohl, and Richard J Stanton, and Blair L Strang
December 1992, The Journal of veterinary medical science,
Kadeem Hyde, and Nowshin Sultana, and Andy C Tran, and Narina Bileckaja, and Claire L Donald, and Alain Kohl, and Richard J Stanton, and Blair L Strang
February 1983, FEBS letters,
Copied contents to your clipboard!