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Characterization of Microbial Degradation Products of Steviol Glycosides. 2021
Gert Steurs, and
Nico Moons, and
Luc Van Meervelt, and
Boudewijn Meesschaert, and
Wim Michel De Borggraeve
Division of Molecular Design and Synthesis, Department of Chemistry, KU Leuven, Celestijnenlaan 200f, P.O. Box 2404, 3001 Leuven, Belgium.
Steviol glycosides were subjected to bacteria present in a soil sample collected from a Stevia plantation in Paraguay. During the incubation experiments, next to the aglycon steviol, steviol degradation products were also formed. X-ray analysis and NMR methods in combination with chemical synthesis and GIAO NMR calculations were used to fully characterize the structure of these compounds as a tricyclic ketone and the corresponding reduced form. They were nicknamed monicanone and monicanol. The latter has the (S)-configuration at the alcohol site.
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