-transparent.png){kind=logo}
Porcine pancreatic alpha-amylase (1,4-alpha-D-glucan glucanohydrolase EC 3.2.1.1), a single polypeptide chain, contains nine residues of methionine. Eight different fragments resulting from cleavage of this molecule by cyanogen bromide were characterized. The sequences of six of them have previously been reported. Two missing fragments, CN2 (82 residues) and CN3b1 (76 residues) were purified after breaking of the interpeptidic disulfide bridge and their complete sequence as well as that of the previously purified CN1 peptide (102 residues) are reported here. The location of the three disulfide bridges present in these peptides was determined. Ordering of the carboxymethylated cyanogen bromide fragments was carried out by pulse labeling the amylase chain in vivo. The complete sequence of the porcine pancreatic amylase chain (496 residues) and the location of its five disulfide bridges is presented. Comparison with human and mouse pancreatic and salivary alpha-amylases and with rat pancreatic amylase obtained from the corresponding cDNA nucleotidic sequences shows a high degree of homology between mammalian alpha-amylases.
L Pasero, and
Y Mazzéi-Pierron, and
B Abadie, and
Y Chicheportiche, and
G Marchis-Mouren
January 1981,
Biochimie,
L Pasero, and
Y Mazzéi-Pierron, and
B Abadie, and
Y Chicheportiche, and
G Marchis-Mouren
April 1970,
The Journal of biological chemistry,
L Pasero, and
Y Mazzéi-Pierron, and
B Abadie, and
Y Chicheportiche, and
G Marchis-Mouren
July 1969,
The Journal of biological chemistry,
L Pasero, and
Y Mazzéi-Pierron, and
B Abadie, and
Y Chicheportiche, and
G Marchis-Mouren
December 1981,
FEBS letters,
L Pasero, and
Y Mazzéi-Pierron, and
B Abadie, and
Y Chicheportiche, and
G Marchis-Mouren
December 1986,
The Journal of biological chemistry,
L Pasero, and
Y Mazzéi-Pierron, and
B Abadie, and
Y Chicheportiche, and
G Marchis-Mouren
May 1981,
Proceedings of the National Academy of Sciences of the United States of America,
L Pasero, and
Y Mazzéi-Pierron, and
B Abadie, and
Y Chicheportiche, and
G Marchis-Mouren
February 1983,
Biochimica et biophysica acta,
L Pasero, and
Y Mazzéi-Pierron, and
B Abadie, and
Y Chicheportiche, and
G Marchis-Mouren
December 2000,
Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology,
L Pasero, and
Y Mazzéi-Pierron, and
B Abadie, and
Y Chicheportiche, and
G Marchis-Mouren
April 1985,
Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme,
L Pasero, and
Y Mazzéi-Pierron, and
B Abadie, and
Y Chicheportiche, and
G Marchis-Mouren
July 1992,
Journal of biochemistry,
