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Mercuric chloride (HgCl2) induces in Brown-Norway rats (BN) a B cell polyclonal activation resulting in autoimmune disease. Spleen cells from BN rats injected with HgCl2 were fused with IR983F, a nonsecreting rat myeloma cell line, in order to obtain monoclonal antibodies reacting with autoantigens or IgE-producing hybridomas. After screening for immunoglobulin-producing clones, we found 5% clones with anti-tissue activity, 8% with anti-TNP activity, and 41% secreting IgE. Among the anti-tissue monoclonal antibodies, one recognizes both TNP and mesangial structures of rat normal glomeruli, which could be an as yet unrecognized mechanism of nephrotoxicity. These experiments 1) confirm that HgCl2 induces polyclonal activation, 2) show that the mercury model is of interest to obtain monoclonal IgE and various autoantibodies, and 3) suggest a new possible mechanism of antibody-mediated renal injury.
F Hirsch, and
J Kuhn, and
M Ventura, and
M C Vial, and
G Fournie, and
P Druet
April 1990,
Journal of immunology (Baltimore, Md. : 1950),
F Hirsch, and
J Kuhn, and
M Ventura, and
M C Vial, and
G Fournie, and
P Druet
November 1995,
Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association,
F Hirsch, and
J Kuhn, and
M Ventura, and
M C Vial, and
G Fournie, and
P Druet
November 1994,
Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology,
F Hirsch, and
J Kuhn, and
M Ventura, and
M C Vial, and
G Fournie, and
P Druet
June 1982,
Clinical and experimental immunology,
F Hirsch, and
J Kuhn, and
M Ventura, and
M C Vial, and
G Fournie, and
P Druet
January 1978,
Annales d'immunologie,
F Hirsch, and
J Kuhn, and
M Ventura, and
M C Vial, and
G Fournie, and
P Druet
December 1993,
Clinical immunology and immunopathology,
F Hirsch, and
J Kuhn, and
M Ventura, and
M C Vial, and
G Fournie, and
P Druet
September 1988,
Clinical and experimental immunology,
F Hirsch, and
J Kuhn, and
M Ventura, and
M C Vial, and
G Fournie, and
P Druet
March 2006,
Rheumatology international,
F Hirsch, and
J Kuhn, and
M Ventura, and
M C Vial, and
G Fournie, and
P Druet
November 2003,
Clinical immunology (Orlando, Fla.),
F Hirsch, and
J Kuhn, and
M Ventura, and
M C Vial, and
G Fournie, and
P Druet
October 1998,
Journal of the American Society of Nephrology : JASN,
