Biomaterial Database

Human T-cell hybrids secreting lymphokines: effect of different parent cell clones of the human T-cell acute lymphoblastoid leukaemia line CCRF-CEM. 1986

G Gallagher, and W H Stimson

We have cloned a number of cell lines from the human T-lymphocyte acute lymphoblastoid leukaemia (ALL) line CCFR-CEM, and attempted to construct functionally active human T-lymphocyte hybrids with them. Functional hybrids were generated using only one particular clone, 3H6. The activities found in the supernatants of two of these hybrids, DB1G7 and DB2D10, are described. Supernatant from DB1G7 was found to suppress strongly the migration of normal human peripheral blood mononuclear cells, while that from DB2D10 was shown to inhibit the proliferative response of human T lymphocytes to both phytohaemagglutinin and concanavalin A. There was no cross-reactivity between the two supernatants, confirming the usefulness of the human T-lymphocyte hybrid technique in dissecting human T-lymphocyte function. The successful use of 3H6 is contrasted with the failure of another clone, 2H2, to permit the production of functional hybrids. Problems relating to the use of CCRF-CEM and its clones as parent cell lines in the production of human T-lymphocyte hybrids are discussed.

UI	MeSH Term	Description	Entries
D007945	Leukemia, Lymphoid	Leukemia associated with HYPERPLASIA of the lymphoid tissues and increased numbers of circulating malignant LYMPHOCYTES and lymphoblasts.	Leukemia, Lymphocytic,Lymphocytic Leukemia,Lymphoid Leukemia,Leukemias, Lymphocytic,Leukemias, Lymphoid,Lymphocytic Leukemias,Lymphoid Leukemias
D008222	Lymphokines	Soluble protein factors generated by activated lymphocytes that affect other cells, primarily those involved in cellular immunity.	Lymphocyte Mediators,Mediators, Lymphocyte
D002460	Cell Line	Established cell cultures that have the potential to propagate indefinitely.	Cell Lines,Line, Cell,Lines, Cell
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D006822	Hybrid Cells	Any cell, other than a ZYGOTE, that contains elements (such as NUCLEI and CYTOPLASM) from two or more different cells, usually produced by artificial CELL FUSION.	Somatic Cell Hybrids,Cell Hybrid, Somatic,Cell Hybrids, Somatic,Cell, Hybrid,Cells, Hybrid,Hybrid Cell,Hybrid, Somatic Cell,Hybrids, Somatic Cell,Somatic Cell Hybrid
D006825	Hybridomas	Cells artificially created by fusion of activated lymphocytes with neoplastic cells. The resulting hybrid cells are cloned and produce pure MONOCLONAL ANTIBODIES or T-cell products, identical to those produced by the immunologically competent parent cell.	Hybridoma
D013601	T-Lymphocytes	Lymphocytes responsible for cell-mediated immunity. Two types have been identified - cytotoxic (T-LYMPHOCYTES, CYTOTOXIC) and helper T-lymphocytes (T-LYMPHOCYTES, HELPER-INDUCER). They are formed when lymphocytes circulate through the THYMUS GLAND and differentiate to thymocytes. When exposed to an antigen, they divide rapidly and produce large numbers of new T cells sensitized to that antigen.	T Cell,T Lymphocyte,T-Cells,Thymus-Dependent Lymphocytes,Cell, T,Cells, T,Lymphocyte, T,Lymphocyte, Thymus-Dependent,Lymphocytes, T,Lymphocytes, Thymus-Dependent,T Cells,T Lymphocytes,T-Cell,T-Lymphocyte,Thymus Dependent Lymphocytes,Thymus-Dependent Lymphocyte