-transparent.png){kind=logo}
Glucocorticoids decrease plasminogen activator (PA) activity in HTC rat hepatoma cells by inducing a specific inhibitor of PA activity (PAI). This inhibitor is similar in several biochemical properties to the PAI purified from bovine aortic endothelial cells (BAEs). We have used reverse fibrin autography and antiserum against BAE PAI to establish more fully the biochemical and immunological relationship of these inhibitors. Both inhibitors migrated with an apparent Mr of approximately 50,000, and the activity of both PAIs was stimulated by treatment with SDS suggesting that each of these molecules exists in both an active and a latent form. Antiserum to the BAE PAI immunoprecipitated all of the HTC PAI demonstrable by reverse fibrin autography. Finally, using this antiserum in a functional immunoassay, we have demonstrated that dexamethasone increases both active and latent PAI made by HTC cells. These results indicate that HTC PAI and BAE PAI are antigenically as well as biochemically related molecules.
D J Loskutoff, and
K Roegner, and
L A Erickson, and
R R Schleef, and
A Huttenlocher, and
P L Coleman, and
T D Gelehrter
August 1989,
Experimental eye research,
D J Loskutoff, and
K Roegner, and
L A Erickson, and
R R Schleef, and
A Huttenlocher, and
P L Coleman, and
T D Gelehrter
March 1986,
The Journal of biological chemistry,
D J Loskutoff, and
K Roegner, and
L A Erickson, and
R R Schleef, and
A Huttenlocher, and
P L Coleman, and
T D Gelehrter
June 1984,
The Journal of biological chemistry,
D J Loskutoff, and
K Roegner, and
L A Erickson, and
R R Schleef, and
A Huttenlocher, and
P L Coleman, and
T D Gelehrter
February 1980,
Life sciences,
D J Loskutoff, and
K Roegner, and
L A Erickson, and
R R Schleef, and
A Huttenlocher, and
P L Coleman, and
T D Gelehrter
November 1987,
Biochemical and biophysical research communications,
D J Loskutoff, and
K Roegner, and
L A Erickson, and
R R Schleef, and
A Huttenlocher, and
P L Coleman, and
T D Gelehrter
January 1979,
Nature,
D J Loskutoff, and
K Roegner, and
L A Erickson, and
R R Schleef, and
A Huttenlocher, and
P L Coleman, and
T D Gelehrter
October 1992,
Journal of cellular physiology,
D J Loskutoff, and
K Roegner, and
L A Erickson, and
R R Schleef, and
A Huttenlocher, and
P L Coleman, and
T D Gelehrter
August 1990,
Thrombosis research,
D J Loskutoff, and
K Roegner, and
L A Erickson, and
R R Schleef, and
A Huttenlocher, and
P L Coleman, and
T D Gelehrter
April 1982,
Biochimica et biophysica acta,
D J Loskutoff, and
K Roegner, and
L A Erickson, and
R R Schleef, and
A Huttenlocher, and
P L Coleman, and
T D Gelehrter
July 1991,
The Journal of biological chemistry,
