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The cDNA sequences encoding mature human interleukin 2 (IL2) and beta-interferon (INF beta), respectively, were fused with various translational initiation regions and inserted into two different types of expression vector. The relative levels of expression of the two genes and the functional stability of their respective mRNAs were examined in vivo in Escherichia coli hosts. The addition of the 30-bp sequence, found immediately upstream of the E. coli atpE gene Shine-Dalgarno (SD) sequence, to the translational initiation regions of IL2 and INF beta increased the expression of both these genes by a factor of 6-10. Thus this sequence, which naturally acts within the E. coli atp operon to enhance the translational initiation frequency of the atpE gene, can increase the expression of other genes in E. coli. It may exemplify a specific type of recognition signal for the E. coli translational apparatus.
J E McCarthy, and
W Sebald, and
G Gross, and
R Lammers
January 1987,
Gene,
J E McCarthy, and
W Sebald, and
G Gross, and
R Lammers
April 1999,
The Journal of biological chemistry,
J E McCarthy, and
W Sebald, and
G Gross, and
R Lammers
July 1988,
Molecular microbiology,
J E McCarthy, and
W Sebald, and
G Gross, and
R Lammers
October 1990,
The Journal of biological chemistry,
J E McCarthy, and
W Sebald, and
G Gross, and
R Lammers
October 2009,
Biotechnology and bioengineering,
J E McCarthy, and
W Sebald, and
G Gross, and
R Lammers
November 1991,
FEBS letters,
J E McCarthy, and
W Sebald, and
G Gross, and
R Lammers
June 1989,
Proceedings of the National Academy of Sciences of the United States of America,
J E McCarthy, and
W Sebald, and
G Gross, and
R Lammers
April 1991,
Molecular microbiology,
J E McCarthy, and
W Sebald, and
G Gross, and
R Lammers
September 1988,
Proceedings of the National Academy of Sciences of the United States of America,
J E McCarthy, and
W Sebald, and
G Gross, and
R Lammers
May 1981,
Journal of bacteriology,
