Agility is an important ability for tennis players. To be successful in the rallies, players must perform rapid, multidirectional movements in response to the ball and/or the position of the opponent. For a test to be representative in monitoring agility performance, it should capture a combination of the physical and cognitive agility performance. Considering that literature reports no reliable and valid sport-specific agility test for tennis, the aim of this article was to design and evaluate the measurement properties of a Tennis-specific Agility Test (TAT). To evaluate the TAT, test-retest reliability, concurrent validity, and feasibility were assessed. For reproducibility, a two-way mixed ANOVA was performed. Concurrent validity was assessed using Pearson correlations. A total of 69 tennis players participated in this study of whom 16 competed at the international (22 ± 3.7 years, playing level (Dynamic Rating System): .8 ± .3), 43 at the national (14 ± 1.4 years, playing level: 4.6 ± 1.4), and 10 at the regional level (15 ± 0.8 years, playing level: 4.9 ± 1.1). Test-retest reliability was found to be moderate with an Intra-Class Correlation coefficient (ICC) of .74 (p < .01) and a percentual minimal detectable change (%MDC) of 6.2%. Concurrent validity was found to be moderate by comparison with a recognised agility test, the Spider Drill, which measures only the physical component (.70; p < .01), and by comparison with tennis performance for both boys (r = .67; p < .01) and girls (r = .72; p < .01). The feasibility was high with short time for preparation (five to ten minutes) and time per participant (<5 minutes). In conclusion, the TAT shows promising results for assessing sport-specific agility performance in tennis making it likely to be used in the practical setting.