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A monoclonal anti-actin antibody, 2C9, was used to study the distribution of an actin-like cell-surface antigen (hereafter termed actin) on a lymphoblastoid cell line LA350 and on human peripheral blood lymphocytes. It was determined that 8-40% of LA350 cells and 3-15% of peripheral blood lymphocytes from healthy donors stain specifically with 2C9, almost exclusively on IgM-positive cells. Treatment of cells with 2C9 prior to incubation caused cell-surface actin to first patch and then to cap. Treatment of cells with nonspecific protease caused a loss of surface actin, with reexpression of the marker after 8-12 hr. The expression of LA350 surface actin also increased with DNA synthesis and was demonstrated to be maximal during late G1/early S phase. Thus, this antigen may be a sensitive marker for activated lymphocytes. These studies contribute to our understanding of the expression and distribution of actin-like membrane proteins that may participate in regulatory signals mediated by anti-actin antibody.
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March 1994,
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M A Bach, and
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W T Shearer
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W T Shearer
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M A Bach, and
D E Lewis, and
J E McClure, and
N Parikh, and
H M Rosenblatt, and
W T Shearer
October 1986,
Journal of immunology (Baltimore, Md. : 1950),
M A Bach, and
D E Lewis, and
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N Parikh, and
H M Rosenblatt, and
W T Shearer
April 1990,
Biochemical Society transactions,
M A Bach, and
D E Lewis, and
J E McClure, and
N Parikh, and
H M Rosenblatt, and
W T Shearer
June 1985,
Journal of immunology (Baltimore, Md. : 1950),
M A Bach, and
D E Lewis, and
J E McClure, and
N Parikh, and
H M Rosenblatt, and
W T Shearer
December 1989,
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M A Bach, and
D E Lewis, and
J E McClure, and
N Parikh, and
H M Rosenblatt, and
W T Shearer
October 1992,
The Journal of general virology,
M A Bach, and
D E Lewis, and
J E McClure, and
N Parikh, and
H M Rosenblatt, and
W T Shearer
June 1993,
Cancer research,
